J Periodontal Res：Poly Lactic-Co-Glycolic Acid支架负载编码FGF-2 DNA质粒促进再植牙齿牙周膜的再生

2020-02-09 lishiting MedSci原创

这篇研究的目的是为了评估poly lactic-co-glycolic acid (PLGA)支架负载编码纤维生长因子-2 DNA的质粒(pFGF-2)对体外培养的人牙周膜细胞(hPDLCs)的作用，并评估PLGA/pFGF-2支架促进比格犬牙撕脱模型中牙周膜(PDL)再生的能力。

这篇研究的目的是为了评估poly lactic-co-glycolic acid (PLGA)支架负载编码纤维生长因子-2 DNA的质粒(pFGF-2)对体外培养的人牙周膜细胞(hPDLCs)的作用，并评估PLGA/pFGF-2支架促进比格犬牙撕脱模型中牙周膜(PDL)再生的能力。研究通过静电纺丝法制备PLGA和PLGA/pFGF-2支架。通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察分析支架材料的特点。通过dsDNA HS检测PLGA/pFGF-2支架的质粒释放情况。通过CCK-8法检测hPDLCs在2种支架上的活性和增殖情况，并通过RT-qPCR检测I型胶原和scleraxis的表达变化。将撕脱牙的牙根分别覆盖2种支架材料并再植入比格犬的牙槽窝内。采用HE和Masson染色评估PLGA/pFGF-2支架材料促进PDL再生的效果。结果显示，2种支架材料均可见平滑且均匀的纤维，并且PLGA/pFGF-2支架中可见质粒随机均匀的分布。dsDNA HS分析结果显示，PLGA/pFGF-2支架在超过21天的时间持续稳定的释放pFGF-2 123 ng，未见明显的爆发式释放。与PL

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2020-05-20 ms8435843352634314

#FGF-2#

30 0
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2020-02-10 wodejia-dayu

#acid#

34 0
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2021-01-05 xqptu

#牙周膜#

29 0
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2020-12-27 xlxchina

#CID#

43 0
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2020-09-27 feather89

#PE#

28 0
[GetPortalCommentsPageByObjectIdResponse(id=2044869, encodeId=fa4c204486993, content=<a href='/topic/show?id=efbee385a6' target=_blank style='color:#2F92EE;'>#FGF-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7385, encryptionId=efbee385a6, topicName=FGF-2)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqPdWFricgmt6Dde2ptNxJzD6Y9c4cxpXdTo4pNlVPARAnFdKu6nWIPele9icxApTaFgibq0uVlgRiceQ/132, createdBy=4b8c2500026, createdName=ms8435843352634314, createdTime=Wed May 20 16:57:00 CST 2020, time=2020-05-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1911447, encodeId=44b2191144e87, content=<a href='/topic/show?id=dbda185590' target=_blank style='color:#2F92EE;'>#acid#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1855, encryptionId=dbda185590, topicName=acid)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7ca4300, createdName=wodejia-dayu, createdTime=Mon Feb 10 03:57:00 CST 2020, time=2020-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1755927, encodeId=d7581e559276c, content=<a href='/topic/show?id=30f46e536d7' target=_blank style='color:#2F92EE;'>#牙周膜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67536, encryptionId=30f46e536d7, topicName=牙周膜)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4a6e37060729, createdName=xqptu, createdTime=Tue Jan 05 19:57:00 CST 2021, time=2021-01-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1991618, encodeId=d53e1991618c8, content=<a href='/topic/show?id=f88a4e047f' target=_blank style='color:#2F92EE;'>#CID#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4704, encryptionId=f88a4e047f, topicName=CID)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=570f404, createdName=xlxchina, createdTime=Sun Dec 27 12:57:00 CST 2020, time=2020-12-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1728292, encodeId=4dc21e2829273, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Sun Sep 27 12:57:00 CST 2020, time=2020-09-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1430215, encodeId=010d143021529, content=<a href='/topic/show?id=b58f6e691fc' target=_blank style='color:#2F92EE;'>#牙齿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67691, encryptionId=b58f6e691fc, topicName=牙齿)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Tue Feb 11 03:57:00 CST 2020, time=2020-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1451192, encodeId=50b41451192aa, content=<a href='/topic/show?id=f0476e49582' target=_blank style='color:#2F92EE;'>#牙周#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67495, encryptionId=f0476e49582, topicName=牙周)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=医者仁心, createdTime=Tue Feb 11 03:57:00 CST 2020, time=2020-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1584763, encodeId=aaf21584e632b, content=<a href='/topic/show?id=83f5e9250e9' target=_blank style='color:#2F92EE;'>#编码#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79250, encryptionId=83f5e9250e9, topicName=编码)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b10a17056098, createdName=12498f6em12暂无昵称, createdTime=Tue Feb 11 03:57:00 CST 2020, time=2020-02-11, status=1, ipAttribution=)]
2020-02-11 zhouqu_8

#牙齿#

23 0
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2020-02-11 医者仁心

#牙周#

24 0
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2020-02-11 12498f6em12暂无昵称

#编码#

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J Periodontal Res：Poly Lactic-Co-Glycolic Acid支架负载编码FGF-2 DNA质粒促进再植牙齿牙周膜的再生

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J Periodontal Res：Poly Lactic-Co-Glycolic Acid支架负载编码FGF-2 DNA质粒促进再植牙齿牙周膜的再生

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Nat Commun：突破！科学家成功开发出新版本干细胞或具有强大的治疗潜力