Cell:新技术或可对干细胞重编程的不同阶段进行**定义

2015-01-14 佚名 生物谷

近日,刊登在国际杂志Cell上的一篇研究论文中,来自加州大学洛杉矶分校(University Of California Los Angeles)的研究人员通过研究对干细胞发育提出了新的理解,同时揭示了其在疾病中的新角色;文章中,研究者建立了一种新方法,该方法可以对特殊细胞重编程为干细胞的特殊阶段进行重新定义。 诱导多能性干细胞(iPSCs)类似于人类胚胎干细胞,其具有自我更新,以及分化成为多种

近日,刊登在国际杂志Cell上的一篇研究论文中,来自加州大学洛杉矶分校(University Of California Los Angeles)的研究人员通过研究对干细胞发育提出了新的理解,同时揭示了其在疾病中的新角色;文章中,研究者建立了一种新方法,该方法可以对特殊细胞重编程为干细胞的特殊阶段进行重新定义。

诱导多能性干细胞(iPSCs)类似于人类胚胎干细胞,其具有自我更新,以及分化成为多种机体细胞的能力,研究者Vincent Pasque表示,我们利用时间过程分析的详细数据针对重编程的过程设计出了一系列的线路图,其包括诱导皮肤细胞重编程为iPSCs,随后研究者观察并分析了在单细胞水平上细胞的转变过程。

结果显示,在细胞重编程过程中发生的改变会以一种有序分阶段的方式来发生,而更为重要的是该阶段在所有不同的重编程系统及所有不同的细胞类型都是相同的;研究者Pasque表示,如今我们已经可以确定任何细胞重编程的精确阶段了,本文研究可以帮助我们以一种阶段性的方式来研究干细胞产生的过程。当前很多研究都忽视了干细胞重编程的阶段,但是如今研究人员就可以从宏观和微观角度同时来理解整个过程了。

未来研究人员希望通过更为深入的研究来揭示将细胞重编程为iPSCs的阶段是否和他们所认为的不一样,他们发现,要想逆转胚胎发育阶段的顺序似乎并不简单,而某些阶段可以以期望的顺序来逆转,但是有些阶段实际上却并不会发生逆转。

最后研究者Pasque表示,本文研究显示,细胞并不喜欢从一种特殊类型转变为另外一种类型,相反其会在细胞同一性上不断抵御改变;抵御细胞重编程或将帮助解释为何重编程仅仅会在一小部分细胞中发生。基于以上研究,下一步研究人员将在细胞重编程的特殊阶段分离特殊类型的细胞进行研究,同时研究者也希望后期将可以更好地阐明iPSCs发育的特性。

原始出处

Vincent Pasque6, Jason Tchieu6, Rahul Karnik.X Chromosome Reactivation Dynamics Reveal Stages of Reprogramming to Pluripotency.Cell.2014

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    2015-06-09 维他命
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    2015-01-14 223.104.5.**

    技术发展迅速!

    0

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