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Med Sci Monit：乔松素预处理对缺血再灌注和心律失常后大鼠心肌Cx43蛋白表达的影响

2018-08-12 何右芹 罂粟花

乔松素心肌梗死
关注

心肌梗死常诱发心律失常，缺血再灌注可加重心肌损伤。有研究表明乔松素可缓解脑缺血和降低心肌梗死面积。因此，本研究通过建立大鼠心肌I/R模型并评估乔松素对室性心律失常和缝隙连接蛋白Cx43表达的影响。

背景与目的

心肌梗死常诱发心律失常，缺血再灌注可加重心肌损伤。有研究表明乔松素可缓解脑缺血和降低心肌梗死面积。因此，本研究通过建立大鼠心肌I/R模型并评估乔松素对室性心律失常和缝隙连接蛋白Cx43表达的影响。

方法

将雄性SD大鼠随机分为假手术组、模型组和乔松素(30 mg/kg)预处理组（n=15）。结扎左冠状动脉前降支30 min建立I/R模型。乔松素预处理组于术前10min经静脉注入乔松素。观察并记录缺血前10min、缺血后10min、再灌注30、60、120min的 HR、MAP、RPP和心率失常情况。利用ELISA法测定血浆CK-MB和cTnI水平，随后进行HE染色，用光谱测定法检测Na+-K+ ATP酶和Ca2+-Mg2+ ATP酶的水平，IHC法测定Cx43的表达，Western blot检测Kir2.1蛋白的表达情况。

结  果

模型组大鼠HR、MAP和 RPP明显低于假手术组，乔松素预处理组血浆指数较高。在模型组和乔松素预处理组中，心率失常指数、CK-MB和 cTnI含量较高，而 Na+-K+ATP酶、 Ca2+-Mg2+ATP酶、 Cx43和 Kir2.1 蛋白表达明显降低 (P<0.05)。

结  论

乔松素通过增强Na+-K+ATP酶和 Ca2+-Mg2+ATP酶的活性，上调Cx43 和 Kir2.1蛋白的表达从而缓解I/R大鼠的室性心律失常。

原始出处：

Zhang P， Xu J， Hu W， et al. Effects of Pinocembrin Pretreatment on Connexin 43 (Cx43) Protein Expression After Rat Myocardial Ischemia-Reperfusion and Cardiac Arrhythmia [J] .  Med. Sci. Monit. 2018 Jul 19 ；24DOI：10.12659/MSM.909162

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    2019-02-10 xuyu

    #乔松素#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2003296, encodeId=1d43200329628, content=<a href='/topic/show?id=c5a0233831b' target=_blank style='color:#2F92EE;'>#乔松素#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=23383, encryptionId=c5a0233831b, topicName=乔松素)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8f5b299, createdName=xuyu, createdTime=Sun Feb 10 23:37:00 CST 2019, time=2019-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=341491, encodeId=6135341491aa, content=好好学习天天向上, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Tue Aug 28 18:29:54 CST 2018, time=2018-08-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1532658, encodeId=0be51532658a9, content=<a href='/topic/show?id=f33388695c2' target=_blank style='color:#2F92EE;'>#蛋白表达#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88695, encryptionId=f33388695c2, topicName=蛋白表达)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d79512374813, createdName=xuyu, createdTime=Tue Aug 14 03:37:00 CST 2018, time=2018-08-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609051, encodeId=c1ee160905104, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Aug 14 03:37:00 CST 2018, time=2018-08-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=338310, encodeId=e21a33831005, content=谢谢分享，学习了。, beContent=null, objectType=article, channel=null, level=null, likeNumber=76, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/07/16/3b24606172648eea459125cb74754afe.jpg, createdBy=6c022216608, createdName=liumin1987, createdTime=Mon Aug 13 09:00:41 CST 2018, time=2018-08-13, status=1, ipAttribution=)]
    2018-08-28 zwjnj2

    好好学习天天向上

    0

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    2018-08-14 xuyu

    #蛋白表达#

    0

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    2018-08-14 ms3994565386320060

    #Med#

    0

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    2018-08-13 liumin1987

    谢谢分享，学习了。

    0