检验

订阅

MedSci主页 / 所有栏目 / 检验

CLIN CHEM LAB MED：用于分析和临床验证结直肠癌转移患者循环肿瘤DNA的一种新的基于扩增子的NGS检测方法

2019-11-17 MedSci MedSci原创

NGS检测方法
关注

评估肿瘤RAS/BRAF状态对转移性结直肠癌(mCRC)患者的治疗选择和预后评估具有重要意义。因此，从文库准备和测序中校正误差对于准确分析循环肿瘤DNA (ctDNA)突变至关重要。本研究评估了基于扩增子的新一代测序(NGS)Firefly™的分析和临床性能，该检测方法采用了基于concatemerer的错误纠正策略。

评估肿瘤RAS/BRAF状态对转移性结直肠癌(mCRC)患者的治疗选择和预后评估具有重要意义。因此，从文库准备和测序中校正误差对于准确分析循环肿瘤DNA (ctDNA)突变至关重要。本研究评估了基于扩增子的新一代测序(NGS)Firefly™的分析和临床性能，该检测方法采用了基于concatemerer的错误纠正策略。

利用来自184例mCRC患者的游离DNA (cfDNA)参考标准和cfDNA样本，评价Firefly法对KRAS/NRAS/BRAF/PIK3CA的靶向性。将血浆检测结果与基于 ARMS的PCR检测结果进行比较。对突变丰度低于检测限(LOD)的样品，采用数字PCR(ddPCR)或NGS进行再次检测。

在20 ng cfDNA水平下，等位基因频率(AF)为0.2%的情况下，Firefly法表现出了优越的灵敏度和特异性，检出率可达到98.89%。一般情况下，血浆cfDNA NGS和FFPE ARMS检测结果阳性分别为40.76%和48.37%。两个平台的一致性为80.11%。在治疗前的队列中，基于Firefly™和ARMS检测17个常见外显子基因型发现，血浆和组织的一致性为93.33%，KRAS/NRAS/BRAF/PIK3CA的阳性百分比一致性(PPA)和阴性百分比一致性(NPA)分别为100%和99.60%。

研究表明，Firefly检测到的总血浆cfDNA为CRC患者的突变谱分析提供了一个可行的补充，它与匹配的肿瘤样本高度一致。总之，这些数据表明Firefly可以常规应用于mCRC患者的临床应用。

原始出处：

Beili Wang，Shengchao Wu，Analytical and clinical validation of a novel amplicon-based NGS assay for the evaluation of circulating tumor DNA in metastatic colorectal cancer patients

本文系梅斯医学（MedSci）原创编译整理，转载需授权！


版权声明：
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料，版权均属于梅斯医学所有。非经授权，任何媒体、网站或个人不得转载，授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章，或“梅斯号”自媒体发布的文章，仅系出于传递更多信息之目的，本站仅负责审核内容合规，其内容不代表本站立场，本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系，我们将立即进行删除处理。
在此留言
评论区 (6)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1370450, encodeId=5a4913e045018, content=<a href='/topic/show?id=d67212e68d7' target=_blank style='color:#2F92EE;'>#NGS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12768, encryptionId=d67212e68d7, topicName=NGS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e5bc315, createdName=Tommy1949, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453321, encodeId=b7b91453321cd, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1457111, encodeId=6995145e111e1, content=<a href='/topic/show?id=b7e86254347' target=_blank style='color:#2F92EE;'>#检测方法#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62543, encryptionId=b7e86254347, topicName=检测方法)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8fe35841975, createdName=gracezdd, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1484872, encodeId=8c9414848e28c, content=<a href='/topic/show?id=35a050454a5' target=_blank style='color:#2F92EE;'>#循环肿瘤DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50454, encryptionId=35a050454a5, topicName=循环肿瘤DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ac107986109, createdName=ms1948154235210413, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1590345, encodeId=1d841590345f1, content=<a href='/topic/show?id=2c17e154824' target=_blank style='color:#2F92EE;'>#癌转移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71548, encryptionId=2c17e154824, topicName=癌转移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2dc017794099, createdName=linagood, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610127, encodeId=3be0161012e56, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 Tommy1949

    #NGS#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1370450, encodeId=5a4913e045018, content=<a href='/topic/show?id=d67212e68d7' target=_blank style='color:#2F92EE;'>#NGS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12768, encryptionId=d67212e68d7, topicName=NGS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e5bc315, createdName=Tommy1949, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453321, encodeId=b7b91453321cd, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1457111, encodeId=6995145e111e1, content=<a href='/topic/show?id=b7e86254347' target=_blank style='color:#2F92EE;'>#检测方法#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62543, encryptionId=b7e86254347, topicName=检测方法)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8fe35841975, createdName=gracezdd, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1484872, encodeId=8c9414848e28c, content=<a href='/topic/show?id=35a050454a5' target=_blank style='color:#2F92EE;'>#循环肿瘤DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50454, encryptionId=35a050454a5, topicName=循环肿瘤DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ac107986109, createdName=ms1948154235210413, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1590345, encodeId=1d841590345f1, content=<a href='/topic/show?id=2c17e154824' target=_blank style='color:#2F92EE;'>#癌转移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71548, encryptionId=2c17e154824, topicName=癌转移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2dc017794099, createdName=linagood, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610127, encodeId=3be0161012e56, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 crystal0564

    #结直肠#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1370450, encodeId=5a4913e045018, content=<a href='/topic/show?id=d67212e68d7' target=_blank style='color:#2F92EE;'>#NGS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12768, encryptionId=d67212e68d7, topicName=NGS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e5bc315, createdName=Tommy1949, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453321, encodeId=b7b91453321cd, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1457111, encodeId=6995145e111e1, content=<a href='/topic/show?id=b7e86254347' target=_blank style='color:#2F92EE;'>#检测方法#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62543, encryptionId=b7e86254347, topicName=检测方法)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8fe35841975, createdName=gracezdd, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1484872, encodeId=8c9414848e28c, content=<a href='/topic/show?id=35a050454a5' target=_blank style='color:#2F92EE;'>#循环肿瘤DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50454, encryptionId=35a050454a5, topicName=循环肿瘤DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ac107986109, createdName=ms1948154235210413, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1590345, encodeId=1d841590345f1, content=<a href='/topic/show?id=2c17e154824' target=_blank style='color:#2F92EE;'>#癌转移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71548, encryptionId=2c17e154824, topicName=癌转移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2dc017794099, createdName=linagood, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610127, encodeId=3be0161012e56, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 gracezdd

    #检测方法#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1370450, encodeId=5a4913e045018, content=<a href='/topic/show?id=d67212e68d7' target=_blank style='color:#2F92EE;'>#NGS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12768, encryptionId=d67212e68d7, topicName=NGS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e5bc315, createdName=Tommy1949, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453321, encodeId=b7b91453321cd, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1457111, encodeId=6995145e111e1, content=<a href='/topic/show?id=b7e86254347' target=_blank style='color:#2F92EE;'>#检测方法#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62543, encryptionId=b7e86254347, topicName=检测方法)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8fe35841975, createdName=gracezdd, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1484872, encodeId=8c9414848e28c, content=<a href='/topic/show?id=35a050454a5' target=_blank style='color:#2F92EE;'>#循环肿瘤DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50454, encryptionId=35a050454a5, topicName=循环肿瘤DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ac107986109, createdName=ms1948154235210413, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1590345, encodeId=1d841590345f1, content=<a href='/topic/show?id=2c17e154824' target=_blank style='color:#2F92EE;'>#癌转移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71548, encryptionId=2c17e154824, topicName=癌转移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2dc017794099, createdName=linagood, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610127, encodeId=3be0161012e56, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 ms1948154235210413

    #循环肿瘤DNA#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1370450, encodeId=5a4913e045018, content=<a href='/topic/show?id=d67212e68d7' target=_blank style='color:#2F92EE;'>#NGS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12768, encryptionId=d67212e68d7, topicName=NGS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e5bc315, createdName=Tommy1949, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453321, encodeId=b7b91453321cd, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1457111, encodeId=6995145e111e1, content=<a href='/topic/show?id=b7e86254347' target=_blank style='color:#2F92EE;'>#检测方法#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62543, encryptionId=b7e86254347, topicName=检测方法)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8fe35841975, createdName=gracezdd, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1484872, encodeId=8c9414848e28c, content=<a href='/topic/show?id=35a050454a5' target=_blank style='color:#2F92EE;'>#循环肿瘤DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50454, encryptionId=35a050454a5, topicName=循环肿瘤DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ac107986109, createdName=ms1948154235210413, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1590345, encodeId=1d841590345f1, content=<a href='/topic/show?id=2c17e154824' target=_blank style='color:#2F92EE;'>#癌转移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71548, encryptionId=2c17e154824, topicName=癌转移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2dc017794099, createdName=linagood, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610127, encodeId=3be0161012e56, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 linagood

    #癌转移#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1370450, encodeId=5a4913e045018, content=<a href='/topic/show?id=d67212e68d7' target=_blank style='color:#2F92EE;'>#NGS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12768, encryptionId=d67212e68d7, topicName=NGS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e5bc315, createdName=Tommy1949, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453321, encodeId=b7b91453321cd, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1457111, encodeId=6995145e111e1, content=<a href='/topic/show?id=b7e86254347' target=_blank style='color:#2F92EE;'>#检测方法#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62543, encryptionId=b7e86254347, topicName=检测方法)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8fe35841975, createdName=gracezdd, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1484872, encodeId=8c9414848e28c, content=<a href='/topic/show?id=35a050454a5' target=_blank style='color:#2F92EE;'>#循环肿瘤DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50454, encryptionId=35a050454a5, topicName=循环肿瘤DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ac107986109, createdName=ms1948154235210413, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1590345, encodeId=1d841590345f1, content=<a href='/topic/show?id=2c17e154824' target=_blank style='color:#2F92EE;'>#癌转移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71548, encryptionId=2c17e154824, topicName=癌转移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2dc017794099, createdName=linagood, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610127, encodeId=3be0161012e56, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:12:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 ms3994565386320060

    #Med#

    0