Exp Eye Res:光谱域光学相干断层扫描的电子显微镜结果与猪视网膜组织学的相关性

2018-08-21 cuiguizhong MedSci原创

美国德克萨斯州Temple医学生理学系Scott&White Eye Institute眼科和眼科血管研究项目的Xie W和Zhao M近日在Exp Eye Res发表了一篇文章,介绍了使用光谱域光学相干断层扫描对类似人的视网膜模型-猪视网膜进行研究。

美国德克萨斯州Temple医学生理学系Scott&White Eye Institute眼科和眼科血管研究项目的Xie W和Zhao M近日在Exp Eye Res发表了一篇文章,介绍了使用光谱域光学相干断层扫描对类似人的视网膜模型-猪视网膜进行研究。

光谱域光学相干断层扫描(SD-OCT)可以用作体内视网膜形态学评估的一种非侵入性工具。猪视网膜是一种类似于人视网膜的模型,目前对于SD-OCT与猪视网膜中视网膜组织学的相关性信息有限。

在本文中,他们将SD-OCT上的低反射和超反射带与层状结构的组织学和猪视网膜的细胞成分相关联。用Heidelberg Spectralis HRA + OCT获得SD-OCT图像。使用环氧树脂包埋的组织和透射电子显微镜进行组织学分析。将来自组织切片的显微照片线性缩放,校正组织收缩,与SD-OCT图像进行关联分析。

研究发现,SD-OCT图像与组织形态学数据相关性良好。中-外内核层中的超反射带与水平细胞和相邻双极细胞中丰富的线粒体相关。锥核的浓度对应于外核层外部的相对低反射带。外视网膜中存在3个超反射带对应于:1)外界膜; 2)锥形和杆形椭圆形区域; 3)光感受器外段或视网膜色素上皮(RPE)顶端细胞和RPE的交叉区。

他们认为,这些相关和标准化的SD-OCT数据可用于表征和评估视网膜血管和退行性疾病的体内组织学变化,可以对这种大型动物模型进行新型治疗干预并检测其反应。

原文出处:

Xie, W., et al., Correlation of spectral domain optical coherence tomography with histology and electron microscopy in the porcine retina. Exp Eye Res, 2018. 

https://www.ncbi.nlm.nih.gov/pubmed/30120928

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    2018-09-20 tulenzi
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style='color:#2F92EE;'>#组织学#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=19, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77676, encryptionId=9238e767680, topicName=组织学)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc0372, createdName=bnurmamat, createdTime=Sun Jun 02 04:45:00 CST 2019, time=2019-06-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1270096, encodeId=00b812e0096cf, content=<a href='/topic/show?id=979690e33bd' target=_blank style='color:#2F92EE;'>#视网膜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90733, encryptionId=979690e33bd, topicName=视网膜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=742793, createdName=zutt, createdTime=Thu Aug 23 09:45:00 CST 2018, time=2018-08-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1555284, encodeId=15561555284ad, content=<a href='/topic/show?id=f367681e434' target=_blank style='color:#2F92EE;'>#猪#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=68174, encryptionId=f367681e434, topicName=猪)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=9ec214601917, createdName=muzishouyi, createdTime=Thu Aug 23 09:45:00 CST 2018, time=2018-08-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1585855, encodeId=f549158585560, content=<a href='/topic/show?id=326a28e723e' target=_blank style='color:#2F92EE;'>#光学相干断层扫描#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=28772, encryptionId=326a28e723e, topicName=光学相干断层扫描)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ba7f17300642, createdName=yyanpro@27cn.c, createdTime=Thu Aug 23 09:45:00 CST 2018, time=2018-08-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=339972, encodeId=08c03399e20f, content=谢谢分享学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Tue Aug 21 18:58:22 CST 2018, time=2018-08-21, status=1, ipAttribution=)]
    2018-08-23 zutt
  8. 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createdBy=ba7f17300642, createdName=yyanpro@27cn.c, createdTime=Thu Aug 23 09:45:00 CST 2018, time=2018-08-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=339972, encodeId=08c03399e20f, content=谢谢分享学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Tue Aug 21 18:58:22 CST 2018, time=2018-08-21, status=1, ipAttribution=)]
    2018-08-23 muzishouyi
  9. 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createdBy=ba7f17300642, createdName=yyanpro@27cn.c, createdTime=Thu Aug 23 09:45:00 CST 2018, time=2018-08-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=339972, encodeId=08c03399e20f, content=谢谢分享学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Tue Aug 21 18:58:22 CST 2018, time=2018-08-21, status=1, ipAttribution=)]
  10. 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createdBy=ba7f17300642, createdName=yyanpro@27cn.c, createdTime=Thu Aug 23 09:45:00 CST 2018, time=2018-08-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=339972, encodeId=08c03399e20f, content=谢谢分享学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Tue Aug 21 18:58:22 CST 2018, time=2018-08-21, status=1, ipAttribution=)]
    2018-08-21 清风拂面

    谢谢分享学习

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