Int Arch Allergy Immunol:纤维素粉对人类鼻上皮细胞活性及纤毛搏动频率的影响研究

2018-11-17 AlexYang MedSci原创

纤维素粉(CP)已经报道为一种安全和有效的作为过敏性鼻炎补充治疗的方法。目前在世界范围内,CP已经获得了越来越多的应用,尤其是在中国,然而缺乏CP对正常人类鼻上皮细胞(hNECs)和纤毛功能的研究。最近,有研究人员探索了CP对hNECs活性和纤毛功能的不良影响。研究人员在非AR患者进行鼻内镜手术时获取了组织样本,并对筛窦或鼻甲组织进行了活检。研究人员将细胞进行了分离,并在气液界面(ALI)进行了区

纤维素粉(CP)已经报道为一种安全和有效的作为过敏性鼻炎补充治疗的方法。目前在世界范围内,CP已经获得了越来越多的应用,尤其是在中国,然而缺乏CP对正常人类鼻上皮细胞(hNECs)和纤毛功能的研究。最近,有研究人员探索了CP对hNECs活性和纤毛功能的不良影响。

研究人员在非AR患者进行鼻内镜手术时获取了组织样本,并对筛窦或鼻甲组织进行了活检。研究人员将细胞进行了分离,并在气液界面(ALI)进行了区分,还利用了流式细胞仪和细胞活力测试检测了CP的细胞毒性。另外,还通过使用ALI诱导模型,调查了CP对纤毛波动频率的影响。研究发现,在第3天和第7天时,高浓度CP(2.5 mg/mL)能够显著的减少hNEC的数目(p<0.05)。随着浓度的增加,细胞死亡的数目从第3天道第7天逐步增加。然而,这些影响在低浓度(0.25 mg/mL, p > 0.05)时并不明显。另外,高剂量的CP(2.5mg)能够显著的减少CBF(p<0.05)。与对照相比,在低浓度时(0.25-2.5mg/mL),CP刚开始能够增加hNEC的CBF,但是随后就会减少。

最后,研究人员指出,高浓度时对细胞毒性和纤毛摆动的抑制证明了在使用CP对AR进行治疗时,应该更加谨慎。

原始出处:

Zhou M, Zuo KJ, Xu ZF et al. Effect of Cellulose Powder on Human Nasal Epithelial Cell Activity and Ciliary Beat Frequency. Int Arch Allergy Immunol. 8 Nov 2018.

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    2018-11-19 膀胱癌
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    2018-11-17 lietome24

    好文,值得点赞!认真学习,应用于实践!谢谢分享给广大同好!

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