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Sci Rep：通过3D打印制备的多隔室支架作为体外共培养成骨模型

2018-10-18 MedSci MedSci原创

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开发体外3D模型以深入了解骨再生机制可以加速实验结果向临床的转化，从而降低实验的成本和持续时间。这项工作探索了通过熔融长丝制造技术3D打印的聚（ε-己内酯）（PCL）中多隔室结构的设计和制造。 该构建体设计有互连的隔室以容纳干细胞和内皮细胞。将细胞包封在优化的结冷胶（GG）基水凝胶基质中，使用锶（Sr2 +）离子交联以利用其生物活性，最后在不同大小的隔室内组装。钙（Ca2 +）-交联凝胶也用

开发体外3D模型以深入了解骨再生机制可以加速实验结果向临床的转化，从而降低实验的成本和持续时间。这项工作探索了通过熔融长丝制造技术3D打印的聚（ε-己内酯）（PCL）中多隔室结构的设计和制造。

该构建体设计有互连的隔室以容纳干细胞和内皮细胞。将细胞包封在优化的结冷胶（GG）基水凝胶基质中，使用锶（Sr2 +）离子交联以利用其生物活性，最后在不同大小的隔室内组装。钙（Ca2 +）-交联凝胶也用作比较Sr2 +成骨作用的对照组。

结果显示，Sr 2+离子在水凝胶基质内成功扩散并且在压缩载荷下增加水凝胶基质的强度。人TERT间充质干细胞（TERT-hMSCs）和人脐静脉内皮细胞（HUVECs）的体外共培养，包裹在含有GG-水凝胶的Sr2 +离子中，并在成骨条件下通过分隔支架相互连接，增强细胞活力。并且支持骨生成，碱性磷酸酶活性显着增加，骨桥蛋白和骨钙素与Ca2 +交联的GG-PCL支架相关。

综上所述，这些结果表明，TERT-hMSCs和HUVEC区室化共培养物的设计和制造使得能够有效地研究和促进骨生成。

原始出处：

Elvira De Giglio, Maria A. Bonifacio, et al., Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model. Sci Rep. 2018; 8: 15130.

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#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1336077, encodeId=1b9613360e764, content=<a href='/topic/show?id=792612047e' target=_blank style='color:#2F92EE;'>#3D#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1204, encryptionId=792612047e, topicName=3D)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/pSGl6CJeZ2PzWxdhCtrvv4U9Q281RV8e0AKmlB5ib93DibbiaRyOzmkCdz5F04cbfX5RGOpG4dOXvuQW1fumibVeBg/132, createdBy=fc342500110, createdName=ms9833124466084992, createdTime=Sat Oct 20 06:01:00 CST 2018, time=2018-10-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351868, encodeId=a0cf13518689b, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Sat Oct 20 06:01:00 CST 2018, time=2018-10-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1047623, encodeId=daab104e623b2, content=梅斯里提供了很多疾病的模型计算公式，赞一个！, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Oct 18 18:01:00 CST 2018, time=2018-10-18, status=1, ipAttribution=)]
    2018-10-20 ms9833124466084992

    #3D#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1336077, encodeId=1b9613360e764, content=<a href='/topic/show?id=792612047e' target=_blank style='color:#2F92EE;'>#3D#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1204, encryptionId=792612047e, topicName=3D)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/pSGl6CJeZ2PzWxdhCtrvv4U9Q281RV8e0AKmlB5ib93DibbiaRyOzmkCdz5F04cbfX5RGOpG4dOXvuQW1fumibVeBg/132, createdBy=fc342500110, createdName=ms9833124466084992, createdTime=Sat Oct 20 06:01:00 CST 2018, time=2018-10-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351868, encodeId=a0cf13518689b, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Sat Oct 20 06:01:00 CST 2018, time=2018-10-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1047623, encodeId=daab104e623b2, content=梅斯里提供了很多疾病的模型计算公式，赞一个！, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Oct 18 18:01:00 CST 2018, time=2018-10-18, status=1, ipAttribution=)]
    2018-10-20 zhaojie88

    #成骨#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1336077, encodeId=1b9613360e764, content=<a href='/topic/show?id=792612047e' target=_blank style='color:#2F92EE;'>#3D#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1204, encryptionId=792612047e, topicName=3D)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/pSGl6CJeZ2PzWxdhCtrvv4U9Q281RV8e0AKmlB5ib93DibbiaRyOzmkCdz5F04cbfX5RGOpG4dOXvuQW1fumibVeBg/132, createdBy=fc342500110, createdName=ms9833124466084992, createdTime=Sat Oct 20 06:01:00 CST 2018, time=2018-10-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351868, encodeId=a0cf13518689b, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Sat Oct 20 06:01:00 CST 2018, time=2018-10-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1047623, encodeId=daab104e623b2, content=梅斯里提供了很多疾病的模型计算公式，赞一个！, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Oct 18 18:01:00 CST 2018, time=2018-10-18, status=1, ipAttribution=)]
    2018-10-18 CHANGE

    梅斯里提供了很多疾病的模型计算公式，赞一个！

    0