J Periodontal Res:衰老的人牙龈成纤维细胞的炎症,增殖和细胞骨架变化

2020-06-30 网络 网络

衰老的特征是组织结构和功能的下降,这可以通过细胞衰老的发展来解释。但是,对衰老的牙龈成纤维细胞中特定表型反应仍知之甚少。本研究分析了人牙龈成纤维细胞原代培养的增殖是否会影响细胞功能。

衰老的特征是组织结构和功能的下降,这可以通过细胞衰老的发展来解释。但是,对衰老的牙龈成纤维细胞中特定表型反应仍知之甚少。本研究分析了人牙龈成纤维细胞原代培养的增殖是否会影响细胞衰老和组织退化有关的不同细胞功能。

收集5个年轻供体的人牙龈成纤维细胞进行培养,直至细胞衰老。通过确定细胞大小,细胞增殖,p16和p21 mRNA水平,H2Ax磷酸化,细胞活力以及衰老相关的β-半乳糖苷酶染色来评估细胞的衰老。通过分析NF-κB的细胞因子分泌和核转位来评估炎症水平。使用胶原蛋白凝胶收缩测定法评估胶原蛋白重塑。使用免疫荧光和共聚焦显微镜确定细胞骨架蛋白的定位变化。使用配对样本t检验或Wilcoxon配对配对秩和检验,进行数据分析以鉴定同一供体在早期传代和晚期传代之间的变化。

结果发现,晚期传代细胞表现出与细胞衰老相关的变化,包括细胞大小增加,细胞增殖减少,SA-βgal染色,磷酸化的H2Ax以及p16和p21 mRNA水平增加。晚期传代细胞显示胶原蛋白收缩减少,并且细胞骨架蛋白肌动蛋白和纽蛋白之间的共定位降低。重要的是,晚期传代细胞没有炎性细胞因子分泌的变化和NF-κB的激活。

细胞骨架的变化和细胞增殖的抑制表明衰老的牙龈成纤维细胞的结构和功能的早期修饰,而这些修饰与炎症性表型的获得不相关。需要进一步的研究来阐明牙周膜衰老过程中不同阶段的变化。

原文出处:

Jassir Páez,Uncoupled inflammatory, proliferative, and cytoskeletal responses in senescent human gingival fibroblasts. Journal of periodontal research, 2020 June. Doi: 10.1111/jre.12727

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