Biomater Sci:ILK/p38通路在介导骨髓间充质干细胞在非晶碳涂层上的成骨分化增强中的作用

2019-01-09 MedSci MedSci原创

无定形碳(a-C)膜是金属植入物表面涂层的有希望的候选物,可改善体内的耐腐蚀性和成骨性。然而,生物惰性碳如何诱导成骨的分子机制尚未阐明。在本研究中,通过真空蒸发器将a-C膜沉积在光滑的纯钛(Ti)植入物表面(C-Ti)上。扫描电子显微镜(SEM)观察显示在C-Ti表面上具有分散颗粒的均匀涂层。拉曼光谱揭示了标准的a-C移动带。在不同的植入物表面上培养大鼠骨髓间充质干细胞(MSC)。与其他研究一致,

无定形碳(a-C)膜是金属植入物表面涂层的有希望的候选物,可改善体内的耐腐蚀性和成骨性。然而,生物惰性碳如何诱导成骨的分子机制尚未阐明。

在本研究中,通过真空蒸发器将a-C膜沉积在光滑的纯钛(Ti)植入物表面(C-Ti)上。扫描电子显微镜(SEM)观察显示在C-Ti表面上具有分散颗粒的均匀涂层。拉曼光谱揭示了标准的a-C移动带。在不同的植入物表面上培养大鼠骨髓间充质干细胞(MSC)。与其他研究一致,C-Ti表面可增强的细胞粘附,增殖和成骨分化。此外,有丝分裂原激活蛋白激酶(MAPK)信号通路的蛋白质印迹分析显示,只有p38磷酸化水平在C-Ti表面上显著增加。SB203580对p38的抑制消除了C-Ti表面的增强的成骨分化能力。此外,在最初的三天内,整联蛋白连接的激酶(ILK)在C-Ti表面上显著上调。siRNA对ILK的特异性敲低明显降低了p38磷酸化,并且还导致C-Ti表面上的成骨分化减少。

最重要的是,我们的研究表明,a-C涂层能够通过ILK/p38信号通路促进MSCs的成骨分化。

原始出处:

Yue G, Song W, et al., Role of ILK/p38 pathway in mediating the enhanced osteogenic differentiation of bone marrow mesenchymal stem cells on amorphous carbon coating. Biomater Sci. 2019 Jan 2. doi: 10.1039/c8bm01151f. 

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    2019-06-06 HinsMax
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    2019-08-08 sunylz
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    2019-01-10 zhaojie88