Int J Mol Med:无远端同源框5是人间充质干细胞成骨的主要调节因子

2019-02-09 MedSci MedSci原创

间充质干细胞(MSC)分化成多个谱系并且是临床使用的有希望的细胞来源。以前,我们发现基因远端的同源框5(DLX5)在具有成骨潜能的MSC中特异性表达。了解成骨机制对于使用MSC成功进行骨再生是必要的。本研究旨在检查DLX5基因在骨生成(骨发育)过程中在MSCs中的功能。我们分析了DLX5表达与从骨髓和脐带血分离的不同细胞中的成骨,软骨形成和脂肪生成相关基因表达之间可能的关联。通过观察形态变化,监测

间充质干细胞(MSC)分化成多个谱系并且是临床使用的有希望的细胞来源。以前,我们发现基因远端的同源框5(DLX5)在具有成骨潜能的MSC中特异性表达。了解成骨机制对于使用MSC成功进行骨再生是必要的。本研究旨在检查DLX5基因在骨生成(骨发育)过程中在MSCs中的功能。

我们分析了DLX5表达与从骨髓和脐带血分离的不同细胞中的成骨,软骨形成和脂肪生成相关基因表达之间可能的关联。通过观察形态变化,监测基因表达模式以及用Von Kossa,番红O和油红O染色来评估分化能力。通过小干扰RNA(siRNA)下调成骨标记抑制DLX5表达并减少其迹象钙矿化。丹参酮IIA是已知的骨形态发生蛋白(BMP)信号传导的小分子激活剂。

本研究中,我们发现MSC中丹参酮IIA对DLX5的诱导增强了成骨分化。此外,我们发现丹参酮IIA(作为BMP2信号传导的介质)激活MSCs中的runt相关转录因子2(RUNX2)并在成骨过程中启动钙矿化。

总之,这些发现表明,在MSC中,DLX5是成骨的主要调节因子。此外,丹参酮IIA对于某些骨病的基于干细胞的疗法可能是有价值的。

原始出处:

June Seok Heo, Seung Gwan Lee, Hyun Ok Kim. Distal-less homeobox 5 is a master regulator of the osteogenesis of human mesenchymal stem cells. Int J Mol Med. 2017 Nov; 40(5): 1486–1494.

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    2019-02-11 zhaojie88
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