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J Dent Res：Purinergic信号调节人牙髓干细胞的存活/增殖

2018-12-07 lishiting MedSci原创

Purinergic信号DPSC增殖存活
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人牙髓干细胞(hDPSCs)存在于出生后的牙髓中，它具有分化为成牙本质细胞和神经元的潜力。然而，细胞间信号龛存在的必要性对hDPSC存活和自我更新的作用还未可知。这篇研究的目的是为了检测hDPSC是否存在细胞间purinergic信号，并评估细胞间purinergic信号对hDPSC存活和增殖的影响。

人牙髓干细胞(hDPSCs)存在于出生后的牙髓中，它具有分化为成牙本质细胞和神经元的潜力。然而，细胞间信号龛存在的必要性对hDPSC存活和自我更新的作用还未可知。这篇研究的目的是为了检测hDPSC是否存在细胞间purinergic信号，并评估细胞间purinergic信号对hDPSC存活和增殖的影响。研究从人的第三磨牙中分离hDPSCs并培养于MEM。为了检测细胞对施加ATP和purinergic受体阻断剂的反应，通过完整的细胞膜片钳技术记录ATP诱导hDPSCs的电流趋势。采用免疫荧光和酶联免疫组化染色分别检测hDPSCs的purinergic受体表达和ectonucleotidase活性变化。为了检测purinergic信号对hDPSC的影响，培养基中添加purinergic受体拮抗剂和ectonucleotidase抑制剂，分别通过DAPI染色和Ki67免疫荧光染色检测hDPSC的存活和增殖。结果显示，施加ATP诱导hDPSC产生内向电流。P2X和P2Y受体参与了ATP诱导内向电流的产生。研究也检测了hDPSC内NTPDase3的表达和ectonucleotidase的活性。进一

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    2019-03-19 jxrzshh

    #牙髓#

    0

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    2019-09-27 mashirong

    #Purinergic#

    0

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    2019-02-01 fyxzlh

    #Purinergic信号#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1669729, encodeId=9b001669e2975, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Tue Mar 19 18:30:00 CST 2019, time=2019-03-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1792914, encodeId=859f1e92914e4, content=<a href='/topic/show?id=59e8148965a' target=_blank style='color:#2F92EE;'>#Purinergic#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14896, encryptionId=59e8148965a, topicName=Purinergic)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f13d475, createdName=mashirong, createdTime=Fri Sep 27 19:30:00 CST 2019, time=2019-09-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1792915, encodeId=01831e92915a7, content=<a href='/topic/show?id=8a4a1489e0c' target=_blank style='color:#2F92EE;'>#Purinergic信号#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14897, encryptionId=8a4a1489e0c, topicName=Purinergic信号)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c9df476, createdName=fyxzlh, createdTime=Fri Feb 01 04:30:00 CST 2019, time=2019-02-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544894, encodeId=b9981544894e6, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Sun Dec 09 13:30:00 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354896, encodeId=cce235489657, content=学习了长知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 09 09:13:22 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354791, encodeId=3bda354e9143, content=认真学习，不断进步，把经验分享给同好。点赞了！, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Sat Dec 08 09:16:45 CST 2018, time=2018-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354781, encodeId=35a0354e81dd, content=好, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Sat Dec 08 07:00:45 CST 2018, time=2018-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354775, encodeId=8f3b354e7512, content=学习学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=69, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRkia1DP5MpwEgtXo1XhXA11xD6MWKxunAZFXKSNic0fWHUQx83oNHPlRMxxIctM83QKeCLkJpMibibiaC/0, createdBy=7e581734408, createdName=深海的鱼, createdTime=Sat Dec 08 06:28:24 CST 2018, time=2018-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354774, encodeId=41e3354e74fd, content=学习了很有用不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Sat Dec 08 00:36:22 CST 2018, time=2018-12-08, status=1, ipAttribution=)]
    2018-12-09 ms6519009802973839

    #牙髓干细胞#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1669729, encodeId=9b001669e2975, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Tue Mar 19 18:30:00 CST 2019, time=2019-03-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1792914, encodeId=859f1e92914e4, content=<a href='/topic/show?id=59e8148965a' target=_blank style='color:#2F92EE;'>#Purinergic#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14896, encryptionId=59e8148965a, topicName=Purinergic)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f13d475, createdName=mashirong, createdTime=Fri Sep 27 19:30:00 CST 2019, time=2019-09-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1792915, encodeId=01831e92915a7, content=<a href='/topic/show?id=8a4a1489e0c' target=_blank style='color:#2F92EE;'>#Purinergic信号#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14897, encryptionId=8a4a1489e0c, topicName=Purinergic信号)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c9df476, createdName=fyxzlh, createdTime=Fri Feb 01 04:30:00 CST 2019, time=2019-02-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544894, encodeId=b9981544894e6, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Sun Dec 09 13:30:00 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354896, encodeId=cce235489657, content=学习了长知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 09 09:13:22 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354791, encodeId=3bda354e9143, content=认真学习，不断进步，把经验分享给同好。点赞了！, beContent=null, objectType=article, channel=null, level=null, likeNumber=65, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7s7ZCKOmmbBTD6nE7gcd5OZpvyGEzkxBP3S275k5ZJVrsPkotZhBglRHM9rKvcj1OgkssqhcnoPw8zIaMZTF8c/0, createdBy=4c441942088, createdName=ylzr123, createdTime=Sat Dec 08 09:16:45 CST 2018, time=2018-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354781, encodeId=35a0354e81dd, content=好, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6d551998554, createdName=1e1c07bdm01(暂无匿称), createdTime=Sat Dec 08 07:00:45 CST 2018, time=2018-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354775, encodeId=8f3b354e7512, content=学习学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=69, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRkia1DP5MpwEgtXo1XhXA11xD6MWKxunAZFXKSNic0fWHUQx83oNHPlRMxxIctM83QKeCLkJpMibibiaC/0, createdBy=7e581734408, createdName=深海的鱼, createdTime=Sat Dec 08 06:28:24 CST 2018, time=2018-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=354774, encodeId=41e3354e74fd, content=学习了很有用不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Sat Dec 08 00:36:22 CST 2018, time=2018-12-08, status=1, ipAttribution=)]
    2018-12-09 1ddf0692m34(暂无匿称)

    学习了长知识

    0

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    2018-12-08 ylzr123

    认真学习，不断进步，把经验分享给同好。点赞了！

    0

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    2018-12-08 1e1c07bdm01(暂无匿称)

    好

    0

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    2018-12-08 深海的鱼

    学习学习学习

    0

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    2018-12-08 飛歌

    学习了很有用不错

    0