Exp Eye Res:葡萄膜黑色素细胞高表达MMP-8并受MAPK途径调控

2018-07-01 cuiguizhong MedSci原创

美国西奈山医院纽约眼耳医院组织培养中心和眼耳医院眼科的Hu DN近日在Exp Eye Res发表了一篇文章,他们研究了培养的人葡萄膜黑色素细胞(UM)中的组成型和TNF-α诱导的MMP-8表达以及涉及的相关信号通路。

美国西奈山医院纽约眼耳医院组织培养中心和眼耳医院眼科的Hu DN近日在Exp Eye Res发表了一篇文章,他们研究了培养的人葡萄膜黑色素细胞(UM)中的组成型和TNF-α诱导的MMP-8表达以及涉及的相关信号通路。

基质金属蛋白酶(MMP)-8是降解1型胶原蛋白的最有效的基质金属蛋白酶,并且在炎症反应和组织重塑过程中起重要作用。 MMP-8主要由多核白细胞表达,并且在大部分非白细胞中是非组成型表达。

从UM和其他细胞类型中收集条件培养基和细胞。采用ELISA试剂盒、Western印迹法和实时定量RT-PCR的方法,分别检测MMP-8蛋白和mRNA的表达。用ELISA试剂盒和western blot检测磷酸化p38 MAPK、ERK1/2和JNK1/2。

在11个UM细胞系和3个无血清培养的葡萄膜黑素瘤细胞系的条件培养基和细胞裂解物中检测到非常高水平的MMP-8蛋白和mRNA,但在来自其他眼睛固有细胞或除了皮肤黑素瘤细胞系的12个恶性细胞系的培养基或细胞裂解物中都没有检测到MMP-8。MMP-8 mRNA和蛋白水平的提高是以TNF-α剂量和时间依赖性方式增加,同时伴随着细胞裂解物中磷酸化的JNK1/2和ERK1/2的显着增加。使用ERK1 / 2(U0126)和JNK1 / 2(SP600125)抑制剂处理后,显着阻断了UM中TNF-α诱导的MMP-8表达和MMP-8组成性表达。

这是关于UM和葡萄膜黑色素瘤细胞表达和分泌MMP-8的第一次研究结果。数据表明,UM可能通过分泌MMP-8,在眼睛炎症相关疾病的重塑过程或发病机制中起作用。

原文出处:

Hu, D.N., et al., Uveal melanocytes express high constitutive levels of MMP-8 which can be upregulated by TNF-alpha via the MAPK pathway. Exp Eye Res, 2018.

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    2019-02-03 sunylz
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    2018-11-05 lidong51
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