Tissue Eng Regen Med：D-RADA16-RGD增强纳米羟基磷灰石/聚酰胺66三元生物材料用于骨形成

2019-04-24 不详网络

纳米羟基磷灰石/聚酰胺66（nHA/PA66）是一种广泛用于修复骨缺损的复合材料。然而，这种材料的生物活性不足。相反，D-RADA16-RGD自组装肽（含有所有D-氨基酸的D-RADA16-RGD序列是Ac-RADARADARADARADARGDS-CONH2）对细胞培养和骨再生都显示出令人兴奋的生物活性。在这里，我们描述了有利的生物材料材料（nHA/PA66/D-RADA16-RGD）的制备。使

纳米羟基磷灰石/聚酰胺66（nHA/PA66）是一种广泛用于修复骨缺损的复合材料。然而，这种材料的生物活性不足。相反，D-RADA16-RGD自组装肽（含有所有D-氨基酸的D-RADA16-RGD序列是Ac-RADARADARADARADARGDS-CONH2）对细胞培养和骨再生都显示出令人兴奋的生物活性。在这里，我们描述了有利的生物材料材料（nHA/PA66/D-RADA16-RGD）的制备。

使用蛋白酶K和圆二色谱测试肽D-RADA16-RGD的稳定性和二级结构性质。扫描电子显微镜（SEM）和透射电子显微镜（TEM）用于表征这些材料的表面。体外研究使用共聚焦激光扫描（CLS），细胞计数试剂盒-8测试（CCK-8），茜素红S染色，细胞免疫荧光分析和蛋白质印迹。此外，研究还评估了材料的生物安全性和生物活性。

结果显示，蛋白酶K和圆二色谱分析表明，nHA/PA66中的D-RADA16-RGD能够形成稳定的二级结构。SEM和TEM显示D-RADA16-RGD材料的宽度为7-33nm，长度为130-600nm，交织的孔径为40-200nm。CLS表明nHA/PA66/D-RADA16-RGD组中的细胞与具有更多肌动蛋白丝的相邻细胞相连。CCK-8分析显示nHA/PA66/D-RADA16-RGD显示出良好的生物相容性。茜素红S染色和Western印迹以及体内成骨的结果表明nHA/PA66/D-RADA16-RGD表现出更好的生物活性。

总之，该研究表明nHA/PA66/D-RADA16-RGD复合材料具有合理的机械性能，生物相容性和生物活性，可促进骨形成。

原始出处：

Zhao W, He B, et al., D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation. Tissue Eng Regen Med. 2019 Jan 5;16(2):177-189. doi: 10.1007/s13770-018-0171-5. eCollection 2019 Apr.

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2019-12-31 1249857fm32暂无昵称

#生物材#

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2019-07-16 xuyu

#Tissue#

24 0
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2020-03-09 dinorsor524

#纳米羟基磷灰石#

34 0
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2019-05-28 zwjnj2

努力学习天天学习天天学习

64 0
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2019-09-09 spoonycyy

#Eng#

23 0
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2019-04-26 般若傻瓜

#ADA#

31 0
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2019-04-26 dongjia2013

#生物材料#

37 0
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2019-04-26 ms3994565386320060

#Med#

24 0
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2019-04-26 爆笑小医

#羟基磷灰石#

38 0

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Tissue Eng Regen Med：D-RADA16-RGD增强纳米羟基磷灰石/聚酰胺66三元生物材料用于骨形成

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