Int Endod J.:低浓度次氯酸钠联合小功率Er,Cr:YSGG激光对粪肠球杆菌的抗菌效果

2015-03-29 MedSci MedSci原创

Int Endod J.:低浓度次氯酸钠联合小功率Er,Cr:YSGG激光对粪肠球杆菌的抗菌效果关键词:次氯酸钠 激光激活冲洗 生物膜 根管消毒 牙髓病学目的:离体牙上研究低浓度次氯酸钠联合小功率Er,Cr:YSGG激光激活冲洗对粪肠球杆菌生物膜的抗菌效果。方法:收集96颗单根管人类牙齿,去除牙冠,根管预备至40号,距离根尖1㎜时根管的锥度为0.06。将样本放置流式盒内灭菌,然后注入营养物质。流式

目的:离体牙上研究低浓度次氯酸钠联合小功率Er,Cr:YSGG激光激活冲洗对粪肠球杆菌生物膜的抗菌效果。

方法:收集96颗单根管人类牙齿,去除牙冠,根管预备至40号,距离根尖1㎜时根管的锥度为0.06。将样本放置流式盒内灭菌,然后注入营养物质。流式盒内接种了粪肠球杆菌(ATCC 700802),培养4周。封闭根尖,将样本分为六个组:1组为对照组,注射器冲洗(SI)根管,针头为27号Monoject针,针头距离根尖1㎜,冲洗两分钟,冲洗液为生理盐水;2组处理过程与1组相同,但冲洗液换成1% NaOCl;3组处理过程与1组相同,但冲洗液换成 4% NaOCl;4组使用0.5% NaOCl冲洗15秒,然后使用激光激活冲洗(LAI)4次,每次15秒;5组处理过程与第4组相同,但是冲洗液换成1% NaOCl;6组处理过程与第4组相同,但是冲洗液换成4% NaOCl。经过这些处理后,将牙齿粉碎,使用串联稀释和电镀法对活菌进行定量检测。使用单因素ANOVA和校正后Turkey post-hoc检验对比各组之间菌落形成单位数量的差别。双尾P值<0.05,认为差异有统计学意义。

结果:1% NaOCl SI组菌落形成单位平均数值明显高于4% NaOCl LAI 组(P=0.02)。

结论:由于本实验的局限性,并未发现低功率(0.05W)Er,Cr:YSGG激光激活冲洗可有效提高低浓度次氯酸钠的抗菌效果。


原始出处:

Christo JE, Zilm PS, Sullivan T, Cathro PR. Efficacy of low concentrations of sodium hypochlorite an low powered Er,Cr:YSGG laser activated irrigationagainst an Enterococcus faecalis biofilm. Int Endod J. 2015 Mar 13. doi: 10.1111/iej.12447.

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    2016-09-13 1e10c84am36(暂无匿称)

    好文章,还会关注

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    2015-03-31 oliver169
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