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Nanomaterials (Basel)：3D水凝胶表型对hMSC分化的影响

2019-04-25 不详 网络

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本研究解决了材料结构和机械负荷对位于由纳米纤维/水凝胶层压复合材料组成的3D体外模型界面的人间充质干细胞（hMSC）的影响。结果显示，在7天的时间内，hMSC表型受到两种刺激的影响。无论环境如何，细胞都平行于下面的纤维方向走向（电纺2D纤维片或具有胶原凝胶层的层压2D片）。施加循环张力（5％应变，1Hz，每天1小时）促使hMSC保留在纤维/凝胶界面，而在静态条件下培养的细胞从界面迁移到上部水凝胶层

本研究解决了材料结构和机械负荷对位于由纳米纤维/水凝胶层压复合材料组成的3D体外模型界面的人间充质干细胞（hMSC）的影响。

结果显示，在7天的时间内，hMSC表型受到两种刺激的影响。无论环境如何，细胞都平行于下面的纤维方向走向（电纺2D纤维片或具有胶原凝胶层的层压2D片）。施加循环张力（5％应变，1Hz，每天1小时）促使hMSC保留在纤维/凝胶界面，而在静态条件下培养的细胞从界面迁移到上部水凝胶层。取决于所应用的刺激，hMSC呈现基因表达的上调，指示几种细胞谱系，在界面处培养并且物理刺激表达指示血管生成，骨生成和成骨的标志物的表达上调。

该研究强调了开发具有特殊表面结构的生物材料支架的重要性，以特异性地将细胞驱向用于生物工程的组织。

原始出处：

Kumar D, Cain SA, Bosworth LA. Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model. Nanomaterials (Basel). 2019 Apr 3;9(4). pii: E522. doi: 10.3390/nano9040522.

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    2019-04-27 tastas

    #水凝胶#

    0

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    2019-04-27 ms9833124466084992

    #3D#

    0

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    2019-04-27 huanbaofeng

    #MSC#

    0

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    2019-04-27 lishizhe

    #凝胶#

    0

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    2019-04-27 tastas

    #表型#

    0