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J Endod：通过二代测序解读牙髓的微生物菌落

2018-06-13 lishiting MedSci原创

菌斑生物膜微生物测序
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生物膜存在于超过70%罹患牙髓疾病的牙齿。通过二代测序(NGS)技术的提高，微生物的研究已经达到对人体宿主内寄生的微生物菌落的深层剖析。在此，本文回顾了使用NGS检测根管内微生物菌落模式的研究。

生物膜存在于超过70%罹患牙髓疾病的牙齿。通过二代测序(NGS)技术的提高，微生物的研究已经达到对人体宿主内寄生的微生物菌落的深层剖析。在此，本文回顾了使用NGS检测根管内微生物菌落模式的研究。本文总共鉴定并认真回顾分析了PubMed上的 12篇同行评审文章。研究条件如下：NGS平台、测序的细菌高变区、包含患者信息的牙齿诊断、样本特征、收集方法和微生物标记。结果显示，最常用的NGS平台为454焦磷酸测序(Roche Diagnostic Corporation， Risch-Rotkreuz， Switzerland)和基于Illumina的技术(Illumina Inc， San Diego， CA)。测序的高变区域在V1和V6之间。患者和样本人群的年龄在12-76岁之间，有症状和无症状牙齿诊断为带或不带根尖周炎的牙髓坏死。微生物样本是从感染牙髓或离体牙内直接进行处理。含量最多的细菌门类为硬壁菌门，放线菌门，拟杆菌门，变形杆菌门和梭杆菌门。最常检测到的种属为普雷沃氏菌属，梭杆菌属，卟啉单胞菌属，微单胞菌属和链球菌属。另外，还存在值得注意的其他不同分类微生物标记，但是它们在不同的研究中

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    2018-08-13 wjcjjian

    #微生物#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1824929, encodeId=a58e1824929c4, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Mon Aug 13 23:54:00 CST 2018, time=2018-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669679, encodeId=227716696e967, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Wed Dec 05 02:54:00 CST 2018, time=2018-12-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1404914, encodeId=2c931404914a3, content=<a href='/topic/show?id=ab2023868f8' target=_blank style='color:#2F92EE;'>#二代测序#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=23868, encryptionId=ab2023868f8, topicName=二代测序)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/PiajxSqBRaEI6UJPia3iaxjs0Sew5N0AnHNTEXOMmiaqkWX238OAQuqcL7I4kcCiaLkUQZB3mBNq3uVBHuMeuWLSGaw/132, createdBy=47512505687, createdName=wangbingxhy, createdTime=Fri Jun 15 11:54:00 CST 2018, time=2018-06-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=323917, encodeId=9f4e32391e8b, content=学习了.谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=60791943465, createdName=1e0f8808m18(暂无匿称), createdTime=Wed Jun 13 21:00:38 CST 2018, time=2018-06-13, status=1, ipAttribution=)]
    2018-12-05 jxrzshh

    #牙髓#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1824929, encodeId=a58e1824929c4, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Mon Aug 13 23:54:00 CST 2018, time=2018-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669679, encodeId=227716696e967, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Wed Dec 05 02:54:00 CST 2018, time=2018-12-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1404914, encodeId=2c931404914a3, content=<a href='/topic/show?id=ab2023868f8' target=_blank style='color:#2F92EE;'>#二代测序#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=23868, encryptionId=ab2023868f8, topicName=二代测序)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/PiajxSqBRaEI6UJPia3iaxjs0Sew5N0AnHNTEXOMmiaqkWX238OAQuqcL7I4kcCiaLkUQZB3mBNq3uVBHuMeuWLSGaw/132, createdBy=47512505687, createdName=wangbingxhy, createdTime=Fri Jun 15 11:54:00 CST 2018, time=2018-06-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=323917, encodeId=9f4e32391e8b, content=学习了.谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=60791943465, createdName=1e0f8808m18(暂无匿称), createdTime=Wed Jun 13 21:00:38 CST 2018, time=2018-06-13, status=1, ipAttribution=)]
    2018-06-15 wangbingxhy

    #二代测序#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1824929, encodeId=a58e1824929c4, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Mon Aug 13 23:54:00 CST 2018, time=2018-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669679, encodeId=227716696e967, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Wed Dec 05 02:54:00 CST 2018, time=2018-12-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1404914, encodeId=2c931404914a3, content=<a href='/topic/show?id=ab2023868f8' target=_blank style='color:#2F92EE;'>#二代测序#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=23868, encryptionId=ab2023868f8, topicName=二代测序)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/PiajxSqBRaEI6UJPia3iaxjs0Sew5N0AnHNTEXOMmiaqkWX238OAQuqcL7I4kcCiaLkUQZB3mBNq3uVBHuMeuWLSGaw/132, createdBy=47512505687, createdName=wangbingxhy, createdTime=Fri Jun 15 11:54:00 CST 2018, time=2018-06-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=323917, encodeId=9f4e32391e8b, content=学习了.谢谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=60791943465, createdName=1e0f8808m18(暂无匿称), createdTime=Wed Jun 13 21:00:38 CST 2018, time=2018-06-13, status=1, ipAttribution=)]
    2018-06-13 1e0f8808m18(暂无匿称)

    学习了.谢谢分享!

    0

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