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Mater Sci Eng C Mater Biol Appl：无针电纺和离心纺聚聚ε-己内酯支架作为组织工程应用中血小板的载体

2019-02-02 MedSci MedSci原创

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用于组织工程的支架的生物功能化对于改善再生疗法的结果是至关重要的。本研究比较了2D静电纺丝和3D离心旋转支架的血小板功能化对hMSCs成骨潜能的影响。使用静电纺丝和离心纺丝技术由聚-ε-己内酯制备的支架使用五种不同浓度的血小板进行功能化。使用在差异和非差异培养基中培养的hMSCs探究细胞增殖，代谢活性和成骨分化。结果显示，离心纺成纤维的多孔3D结构导致更高的细胞增殖。此外，支架与血小板的功能化导致

用于组织工程的支架的生物功能化对于改善再生疗法的结果是至关重要的。本研究比较了2D静电纺丝和3D离心旋转支架的血小板功能化对hMSCs成骨潜能的影响。

使用静电纺丝和离心纺丝技术由聚-ε-己内酯制备的支架使用五种不同浓度的血小板进行功能化。使用在差异和非差异培养基中培养的hMSCs探究细胞增殖，代谢活性和成骨分化。

结果显示，离心纺成纤维的多孔3D结构导致更高的细胞增殖。此外，支架与血小板的功能化导致细胞代谢活性，增殖和成骨标记物-碱性磷酸酶的产生的剂量依赖性增加。通过在成骨性差异培养基中培养进一步促进了该效果。血小板和成骨培养基组合的增加表明在富含无机磷酸盐和抗坏血酸盐的环境中血小板的骨诱导得到改善。然而，研究结果表明诱导hMSC成骨的血小板最佳浓度范围为900-3000×109血小板/L。

总之，该研究确定了电纺和离心纺粘纤维与粘附血小板的潜力，可用于骨组织工程。

原始出处：

Lukášová V, Buzgo M, et al., Needleless electrospun and centrifugal spun poly-ε-caprolactone scaffolds as a carrier for platelets in tissue engineering applications: A comparative study with hMSCs. Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:567-575. doi: 10.1016/j.msec.2018.12.069. Epub 2018 Dec 21.

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    2019-03-20 sunylz

    #Bio#

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    2019-09-08 12498ebem31暂无昵称

    #Biol#

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    2019-03-20 spoonycyy

    #Eng#

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    2019-03-25 mei539

    #载体#

    0

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    2019-02-04 zhu_jun9838

    #组织工程#

    0