CLIN CHEM LAB MED:生物素干扰:新一代电化学发光免疫分析法检测高灵敏度肌钙蛋白T和促甲状腺激素的评价

2020-11-30 MedSci原创 MedSci原创

对于使用基于生物素-链霉亲和素的免疫测定法的实验室来说,生物素目前是一个令人关注的问题。据报道,生物素干扰素可用于高敏感性肌钙蛋白T(hsTnT)和甲状腺刺激激素(TSH)分析。

对于使用基于生物素-链霉亲和素的免疫测定法的实验室来说,生物素目前是一个令人关注的问题。据报道,生物素干扰素可用于高敏感性肌钙蛋白T(hsTnT)和甲状腺刺激激素(TSH)分析。本研究的目标是评估新一代的hsTnT和TSH电化学发光免疫分析方法(它们对生物素的敏感性较低。)。

首先,研究人员评估了新一代分析方法的分析性能,包括不精确性、偏差、总误差、定量限制,并比较了在没有生物素的情况下,新一代分析方法的分析性能。其次,用加入了七种不同生物素水平的样品来对这两代的检测方法比较。同时,对新一代检测方法的效率也与链霉亲和素珠处理进行了比较。

研究结果显示,新一代的分析方法可提供合适的分析性能。在没有生物素的情况下,前代和新一代的hsTnT和TSH分析都是可互换的。在存在生物素的情况下,研究人员确认了上一代测定受低至40.5 ng / mL的生物素浓度的影响,并且在宣布的耐受阈值达到1200 ng / mL时,新一代测定也没有受到影响。与新一代测定法相比,链霉亲和素珠处理后,观察到两个参数的不准确性更高,TSH的恒定负偏差为10%。

研究结果表明,新一代电致化学发光免疫分析是防止生物素干扰hsTnT和TSH测试的可靠系统解决方案。

原始出处:

Samy MzouguiJulien FavresseBiotin interference: evaluation of a new generation of electrochemiluminescent immunoassays for high-sensitive troponin T and thyroid-stimulating hormone testing

 

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    2021-10-21 kksonne
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    2020-12-02 huagfeg
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