期刊： NATURE PROTOCOLS, 2021; 16 (7)
Glycosphingolipids (GSLs) are ubiquitous glycoconjugates present on the cell membrane; they play significant roles in many bioprocesses such as cell a......
期刊： NATURE PROTOCOLS, 2021; 16 (7)
Cellular heterogeneity is pervasive and of paramount importance in biology. Single-cell analysis techniques are indispensable for understanding the he......
期刊： NATURE PROTOCOLS, 2021; 16 (8)
The integration of DNA methylation and transcriptional state within single cells is of broad interest. Several single-cell dual- and multi-omits appro......
期刊： NATURE PROTOCOLS, 2021; 16 (9)
Solid-state nanochannels (SSNs) provide a promising approach for biosensing due to the confinement of molecules inside, their great mechanical strengt......
期刊： NATURE PROTOCOLS, 2021; 16 (9)
Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of t......
期刊： NATURE PROTOCOLS, 2021; 16 (2)
Cultivating native bacteria from roots of plants grown in a given environment is essential for dissecting the functions of the root microbiota for pla......
期刊： NATURE PROTOCOLS, 2021; 16 (2)
The complexity and dynamics of human diseases are driven by the interactions between internal molecular activities and external environmental exposure......
期刊： NATURE PROTOCOLS, 2021; 16 (9)
Laser scanning is used in advanced biological microscopy to deliver superior imaging contrast, resolution and sensitivity. However, it is challenging ......
期刊： NATURE PROTOCOLS, 2021; 16 (6)
Emerging evidence has demonstrated that RNA-RNA interactions are vital in controlling diverse biological processes, including transcription, RNA splic......
期刊： NATURE PROTOCOLS, 2021; 16 (8)
This protocol describes a plate-based ATAC-seq assay that combines up-front bulk tagging of accessible DNA by the Tn5 transposase with FACS sorting fo......
Reverse genetic systems are a critical tool for studying viruses and identifying countermeasures. In response to the ongoing COVID-19 pandemic, we rec......
The stable maintenance of DNA methylation patterns during mitotic cell division is crucial for cell identity. Precisely determining the maintenance ki......
期刊： NATURE PROTOCOLS, 2020; 15 (5)
neuECG is the simultaneous noninvasive recording of ECG and skin sympathetic nerve activity and thus can directly record sympathetic nerve activity ov......
期刊： NATURE PROTOCOLS, 2020; 15 (6)
The authors demonstrate two variants of the cellular thermal shift assay (CETSA) protocol-the melt curve and the isothermal dose-response-for use on i......
期刊： NATURE PROTOCOLS, 2020; 15 (5)
The HDOCK server is developed for template-based and template-free protein-protein docking, using amino acid sequences or PDB structures as inputs. HD......
