期刊： CRISPR JOURNAL, 2022; 5 (3)
CRISPR-guided DNA base editors (BEs) are potent genome editing tools in biotechnology and medicine. However, conventional cytosine and adenine BEs can......
期刊： CRISPR JOURNAL, 2022; 5 (6)
Genome editing tools have simplified the generation of knock-in gene fusions, which are widely used to study proteins in their natural context. Howeve......
期刊： CRISPR JOURNAL, 2022; 5 (3)
Many therapeutic applications of CRISPR-Cas9 gene editing rely on delivery using the highly versatile adenoassociated virus (AAV) vector. The smallest......
期刊： CRISPR JOURNAL, 2022; 5 (6)
The recently established prime editor (PE) system is regarded as next-generation gene-editing technology. This methodology can install any base-to-bas......
期刊： CRISPR JOURNAL, 2022; 5 (1)
Detection of genome editing with quantitative polymerase chain reaction (PCR) primarily relies on and is limited by its ability to discriminate genome......
期刊： CRISPR JOURNAL, 2022; 5 (6)
The white coat color of Yorkshire pigs is caused by the dominant white I allele, which has been associated with at least one copy of the 450-kb duplic......
期刊： CRISPR JOURNAL, 2022; 5 (1)
The CRISPR-Cas nuclease has emerged as a powerful genome-editing tool in recent years. The CRISPR-Cas system induces double-strand breaks that can be ......
期刊： CRISPR JOURNAL, 2022; 5 (5)
B lymphocytes are activated and regulated by their interactions with T cells, a process that results in one-way class switching of immunoglobulins (ig......
期刊： CRISPR JOURNAL, 2022; 5 (6)
The CRISPR-Cas9 system shows diverse levels of genome editing activities on eukaryotic chromatin, and high-efficiency sgRNA targets are usually desire......
期刊： CRISPR JOURNAL, 2022; 5 (3)
The CRISPR-Cas9 system is increasingly being used as a gene editing therapeutic technique in complex diseases but concerns remain regarding the clinic......
期刊： CRISPR JOURNAL, 2021; 4 (3)
Recombinant adenoviruses have broad applications for gene delivery and expression. Furthermore, the adenovirus packaging system facilitates the expres......
期刊： CRISPR JOURNAL, 2021; 4 (3)
Brassica napus is the most important oil crop plant for edible oil and renewable energy source worldwide. Yield loss caused by pod shattering is a mai......
期刊： CRISPR JOURNAL, 2021; 4 (4)
Gene manipulations of human induced pluripotent stem cells (iPSCs) by CRISPR-Cas9 genome engineering are widely used for disease modeling and regenera......
期刊： CRISPR JOURNAL, 2021; 4 (3)
Since its Nobel Prize-winning breakthrough in 2012, CRISPR-Cas-based gene-editing system has emerged as one of the most promising biotechnologies in d......
