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A Simple Dry Sectioning Method for Obtaining WholeSeed-Sized Resin Section and Its Applications

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (167)

The morphology, size and quantity of cells, starch granules and protein bodies in seed determine the weight and quality of seed. They are significantl......

Establishing Transcription Profile of Psoriasiform Cutaneous In Vitro using HaCaT Cells Stimulated with Combination of Cytokines

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (169)

Psoriasis is a common chronic inflammatory skin disease mediated by innate and adaptive immune systems, characterized by abnormal proliferation and di......

Detecting Amyloid-beta Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (170)

Alzheimer's disease (AD) is a neurodegenerative disease that contributes to 60-70% dementia around the world. One of the hallmarks of AD undoubtedly l......

Individualized rTMS Treatment for Depression using an fMRI-based Targeting Method

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (174)

To achieve greater clinical efficacy, a revolution in treatment for major depressive disorder (MDD) is highly anticipated. Repetitive transcranial mag......

Propagation of Dental and Respiratory Cells and Organs in Microgravity

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (171)

Gravity is one of the key determinants of human cell function, proliferation, cytoskeletal architecture and orientation. Rotary bioreactor systems (RC......

Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (168)

This protocol describes the allotransplantation of tumors in Drosophila melanogaster using an auto-nanoliter injection apparatus. With the use of an a......

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (169)

In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living......

A Fluorescence Assay for Evaluating the Permeability of a Cardiac Microvascular Endothelial Barrier in a Rat Model of Ischemia/reperfusion

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (172)

Revascularization therapies for culprit arteries, regardless of percutaneous coronary intervention and coronary artery bypass grafting, are considered......

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (173)

Imaging neuronal activities at single-cell resolution in awake behaving animals is a very powerful approach for the investigation of neural circuit fu......

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [F-18]FDG Micro-PET/MR Imaging

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (173)

Brown and beige adipocytes are now recognized as potential therapeutic targets for obesity and metabolic syndromes. Non-invasive molecular imaging met......

A non-coding small RNA MicC contributes to virulence in outer membrane proteins in Salmonella Enteritidis

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (167)

A non-coding small RNA (sRNA) is a new factor to regulate gene expression at the post-transcriptional level. A kind of sRNA MicC, known in Escherichia......

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (169)

Diagnosis of the ongoing SARS-CoV-2 pandemic is a priority for all countries across the globe. Currently, reverse transcription quantitative PCR (RT-q......

Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (170)

Escherichia coli (E. coli) is the most common Gram-negative bacteria causing neonatal meningitis. The occurrence of bacteremia and bacterial penetrati......

Interaction between Phonological and Semantic Processes in Visual Word Recognition using Electrophysiology

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (172)

Controversies have always existed in research related to reading abilities; on whether printed words are perceived in a feedforward manner based on or......

An Adjustable High-Definition Imaging System for Behavioral Studies of Drosophila Adults

期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021; (172)

Drosophila melanogaster is a very powerful model in biological research, but a bad model for photography or videography. This paper describes a simple......

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