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Conclusions conclusion

Eur Heart J Cardiovasc Imaging. 2013 July; 14(7): 650–658.

ConclusionsConcentric remodelling is prevalent in patients harbouring the m.3243A>G mutation and occurs in association with characteristic changes in systolic intramyocardial strains and torsion. These findings, which are closely related to urinary mutation load and disease burden, occur in patients without existing evidence of cardiac involvement, and may provide an early marker of myocardial pathology, enabling future studies of pathogenesis and intervention.

MicroRNAs Are Essential for Stretch-induced Vascular Smooth Muscle Contractile Differentiation via MicroRNA (miR)-145-dependent Expression of L-type C methods

J Biol Chem. 2012 June 1; 287(23): 19199–19206.

Vascular smooth muscle cells were isolated from mouse aorta by enzymatic digestion as described previously (18). Passages 3–6 were used for experiments. Cells were transfected with commercially available miR-145 inhibitor (Dharmacon) using Oligofectamine transfection reagent (Invitrogen) as described previously (18). Myocardin was overexpressed using adenoviral transduction as described (18, 31). Briefly, cells were transduced using 100 multiplicity of infection Ad.Myocd or Ad.CMV-Null virus for 96 h in normal growth medium.

Inflammation marker and risk of pancreatic cancer: a nested case–control study within the EPIC cohort methods

Br J Cancer. 2012 May 22; 106(11): 1866–1874.

Follow-up for cancer incidence and vital status In six of the participating countries (Denmark, Italy, the Netherlands, Spain, Sweden, and the United Kingdom), follow-up of cancer cases was based on population registries. In the other three countries (France, Germany, and Greece), a combination of methods was used including health insurance records, cancer and pathology registries, and active follow-up through study subjects and their next-of-kin. In all EPIC centres, data on vital status are collected from mortality registries at the regional or national level, which is combined with health insurance data (France) or data collected by active follow-up (Greece). Cases reported in this study were all diagnosed up to the latest dates of complete follow-up, which was between December 2002 and 2005, depending on the study centre. For Germany, Greece, and France, the end of follow-up was the last known contact, date of diagnosis, or date of death, whichever came first.

Angiotensin II Receptor Blocker Attenuates Intrarenal Renin-Angiotensin-System and Podocyte Injury in Rats with Myocardial Infarction methods

PLoS One. 2013; 8(6): e67242.

Enzyme-linked immunosorbent assaySamples of tissue homogenate supernatant and blood serum collected at 3 and 9 weeks were tested for AngII, IGF-1 and cystatin C contents. Enzyme-linked immunosorbent assays were performed using commercial kits against AngII (SPI-BIO, France), IGF-1 (MG100, R&D Systems) and cystatin C (MSCTC0, R&D Systems) according to the manufacturer’s instructions.

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