笔记详情
标题
MicroRNAs Are Essential for Stretch-induced Vascular Smooth Muscle Contractile Differentiation via MicroRNA (miR)-145-dependent Expression of L-type C
内容
Vascular smooth muscle cells were isolated from mouse aorta by enzymatic digestion as described previously (18).
Passages 3–6 were used for experiments. Cells were transfected with
commercially available miR-145 inhibitor (Dharmacon) using
Oligofectamine transfection reagent (Invitrogen) as described previously
(18). Myocardin was overexpressed using adenoviral transduction as described (18, 31).
Briefly, cells were transduced using 100 multiplicity of infection
Ad.Myocd or Ad.CMV-Null virus for 96 h in normal growth medium.
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来源
J Biol Chem. 2012 June 1; 287(23): 19199–19206.
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