笔记详情
标题
Tumor generation
内容
Tumor generation
Six-week-old female C57B6 mice were weighed and randomized into the 3 dietary treatment groups as described above: (1) RD (n = 20), (2) HED (n = 20) and (3) CRD (n = 10). Mice were weighed twice a week. After 30 days of respective diet, 5 × 106 ID-8 mouse ovarian cancer cells in 200 μl phosphate-buffered saline were injected into the peritoneal cavity of the mice [13]. Post-7 days of tumor implantation, 1 set of mice from the RD and HED groups (n = 10) was given 200 mg/body weight metformin in water [93] daily, till the end of the study. The mice were monitored daily for any discomfort and weighed twice a week. The diet regimens were continued for another 60 days, after which the mice were sacrificed and autopsied. Ascitic fluid, blood, tumor tissue and vital organs were collected from each mouse.
Tumor score
Tumor nodules morphology and count were identified grossly at the liver, spleen, kidneys, bowel, peritoneum and diaphragm. A scoring system to identify the tumor burden in every organ was used as 0: no nodule observed; 1: 1 nodule observed; 2: 2 to 5 nodules and 3: more than 5 nodules observed per organ [13]. Scoring was performed in a blinded manner by 2 individuals including a gynecology oncology fellow (ZW). 点击翻译
Six-week-old female C57B6 mice were weighed and randomized into the 3 dietary treatment groups as described above: (1) RD (n = 20), (2) HED (n = 20) and (3) CRD (n = 10). Mice were weighed twice a week. After 30 days of respective diet, 5 × 106 ID-8 mouse ovarian cancer cells in 200 μl phosphate-buffered saline were injected into the peritoneal cavity of the mice [13]. Post-7 days of tumor implantation, 1 set of mice from the RD and HED groups (n = 10) was given 200 mg/body weight metformin in water [93] daily, till the end of the study. The mice were monitored daily for any discomfort and weighed twice a week. The diet regimens were continued for another 60 days, after which the mice were sacrificed and autopsied. Ascitic fluid, blood, tumor tissue and vital organs were collected from each mouse.
Tumor score
Tumor nodules morphology and count were identified grossly at the liver, spleen, kidneys, bowel, peritoneum and diaphragm. A scoring system to identify the tumor burden in every organ was used as 0: no nodule observed; 1: 1 nodule observed; 2: 2 to 5 nodules and 3: more than 5 nodules observed per organ [13]. Scoring was performed in a blinded manner by 2 individuals including a gynecology oncology fellow (ZW). 点击翻译
来源
Zaid Al-Wahab. Oncotarget. 2015 Mar 12
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关键词
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