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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1989 2
1990 16
1991 26
1992 32
1993 16
1994 19
1995 23
1996 5
2001 4
2002 4
2003 4
2004 4
2005 4
2006 17
2007 42
2008 35
2009 24
2010 17
2011 27
2012 15
2013 29
2014 19
2015 19
2016 39
2017 33
2018 25
2019 34
2020 16
2021 15
2022 7
2023 4
2024 1

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577 results

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Page 1
A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector.
Frank SB, Schulz VV, Miranti CK. Frank SB, et al. BMC Biotechnol. 2017 Feb 28;17(1):24. doi: 10.1186/s12896-017-0341-x. BMC Biotechnol. 2017. PMID: 28245848 Free PMC article.
In addition, we generated EZ-Tet-pLKO vectors with hygromycin or blasticidin resistance to provide greater flexibility in cell line engineering. Furthermore, we provide a detailed guide for utilizing these vectors, including shRNA design strategy and simplified screening m …
In addition, we generated EZ-Tet-pLKO vectors with hygromycin or blasticidin resistance to provide greater flexibility in cell line engin …
Targeted genome editing in acute lymphoblastic leukemia: a review.
Montaño A, Forero-Castro M, Hernández-Rivas JM, García-Tuñón I, Benito R. Montaño A, et al. BMC Biotechnol. 2018 Jul 17;18(1):45. doi: 10.1186/s12896-018-0455-9. BMC Biotechnol. 2018. PMID: 30016959 Free PMC article. Review.
These tools not only broaden the understanding of the involvement of various genetic alterations in the pathogenesis of the disease but also identify new therapeutic targets for future clinical trials. CONCLUSIONS: New approaches including CRISPR-Cas9 are crucial for funct …
These tools not only broaden the understanding of the involvement of various genetic alterations in the pathogenesis of the disease b …
Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass.
Luo G, Cao VD, Kannan B, Liu H, Shanklin J, Altpeter F. Luo G, et al. BMC Biotechnol. 2022 Aug 30;22(1):24. doi: 10.1186/s12896-022-00753-7. BMC Biotechnol. 2022. PMID: 36042455 Free PMC article.
BACKGROUND: The metabolic engineering of high-biomass crops for lipid production in their vegetative biomass has recently been proposed as a strategy to elevate energy density and lipid yields for biodiesel production. ...Pearson's correlation analysis showed that the accu …
BACKGROUND: The metabolic engineering of high-biomass crops for lipid production in their vegetative biomass has recently been propos …
Genetic engineering of Escherichia coli to improve L-phenylalanine production.
Liu Y, Xu Y, Ding D, Wen J, Zhu B, Zhang D. Liu Y, et al. BMC Biotechnol. 2018 Jan 30;18(1):5. doi: 10.1186/s12896-018-0418-1. BMC Biotechnol. 2018. PMID: 29382315 Free PMC article.
BACKGROUND: L-phenylalanine (L-Phe) is an essential amino acid for mammals and applications expand into human health and nutritional products. In this study, a system level engineering was conducted to enhance L-Phe biosynthesis in Escherichia coli. ...CONCLUSION: The meta …
BACKGROUND: L-phenylalanine (L-Phe) is an essential amino acid for mammals and applications expand into human health and nutritional product …
Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology.
Dasgupta K, Hotton S, Belknap W, Syed Y, Dardick C, Thilmony R, Thomson JG. Dasgupta K, et al. BMC Biotechnol. 2020 Aug 20;20(1):43. doi: 10.1186/s12896-020-00635-w. BMC Biotechnol. 2020. PMID: 32819338 Free PMC article.
BACKGROUND: Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development. RESULTS: In this study, we report fiv …
BACKGROUND: Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality tra …
Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability.
Zhou Z, Wang X. Zhou Z, et al. BMC Biotechnol. 2021 May 3;21(1):32. doi: 10.1186/s12896-021-00693-8. BMC Biotechnol. 2021. PMID: 33941157 Free PMC article.
Here, we improved the thermostability of an alkaline pectate lyase (PelN) from Paenibacillus sp. 0602 with rational design and structure-based engineering. RESULTS: From 26 mutants, two mutants of G241A and G241V showed a higher thermostability compared with the wild-type …
Here, we improved the thermostability of an alkaline pectate lyase (PelN) from Paenibacillus sp. 0602 with rational design and structure-bas …
Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects.
Zheng G, Zhu Q, Dong J, Lin X, Zhu C. Zheng G, et al. BMC Biotechnol. 2019 Nov 8;19(1):74. doi: 10.1186/s12896-019-0573-z. BMC Biotechnol. 2019. PMID: 31703569 Free PMC article.
BACKGROUND: Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated tool for genetic modifications. However, uti …
BACKGROUND: Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are …
577 results