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The expanding horizons of asparagine-linked glycosylation.
Larkin A, Imperiali B. Larkin A, et al. Biochemistry. 2011 May 31;50(21):4411-26. doi: 10.1021/bi200346n. Epub 2011 May 4. Biochemistry. 2011. PMID: 21506607 Free PMC article. Review.
In the past decade, research in the field of N-linked glycosylation has achieved major advances, including the discovery of new carbohydrate modifications, the biochemical characterization of the enzymes involved in glycan assembly, and the determination of the biol …
In the past decade, research in the field of N-linked glycosylation has achieved major advances, including the discovery of new ca …
Flexible Synthetic Carbohydrate Receptors as Inhibitors of Viral Attachment.
Bravo MF, Lema MA, Marianski M, Braunschweig AB. Bravo MF, et al. Biochemistry. 2021 Apr 6;60(13):999-1018. doi: 10.1021/acs.biochem.0c00732. Epub 2020 Oct 23. Biochemistry. 2021. PMID: 33094998
Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step in the virus life cycle that precedes infection and, ultimately, replication. Despite the conserved structures of the glycans involved in doc …
Carbohydrate-receptor interactions are often involved in the docking of viruses to host cells, and this docking is a necessary step i …
Cooperative Kinetics of the Glucan Phosphatase Starch Excess4.
Mak CA, Weis K, Henao T, Kuchtova A, Chen T, Sharma S, Meekins DA, Thalmann M, Vander Kooi CW, Raththagala M. Mak CA, et al. Biochemistry. 2021 Aug 10;60(31):2425-2435. doi: 10.1021/acs.biochem.1c00307. Epub 2021 Jul 28. Biochemistry. 2021. PMID: 34319705
We investigated the basis for this positive kinetic cooperativity and determined that the SEX4 carbohydrate-binding module (CBM) dramatically influences the binding cooperativity and substrate transformation rates. These findings provide insights into a previously unknown …
We investigated the basis for this positive kinetic cooperativity and determined that the SEX4 carbohydrate-binding module (CBM) dram …
Characterization of a Hybrid Nonribosomal Peptide-Carbohydrate Biosynthetic Pathway in Photorhabdus luminescens.
Perez CE, Crawford JM. Perez CE, et al. Biochemistry. 2019 Feb 26;58(8):1131-1140. doi: 10.1021/acs.biochem.8b01120. Epub 2019 Feb 13. Biochemistry. 2019. PMID: 30694662
Advances in genome sequencing and analysis have afforded a trove of "orphan" bacterial biosynthetic pathways, many of which contain hypothetical proteins. ...We previously identified a rare hybrid nonribosomal peptide synthetase (NRPS)-carbohydrate genomic island in …
Advances in genome sequencing and analysis have afforded a trove of "orphan" bacterial biosynthetic pathways, many of which contain h …
Characterization of a Thermobifida fusca beta-1,3-glucanase (Lam81A) with a potential role in plant biomass degradation.
McGrath CE, Wilson DB. McGrath CE, et al. Biochemistry. 2006 Nov 28;45(47):14094-100. doi: 10.1021/bi061757r. Biochemistry. 2006. PMID: 17115704
In this paper, we report the cloning, expression, purification, and characterization of the T. fusca enzyme, Lam81A. The Carbohydrate Active Enzymes Database (http://afmb.cnrs-mrs.fr/CAZY/) indicates that Lam81A belongs to a relatively uncharacterized family of beta-1,3-gl …
In this paper, we report the cloning, expression, purification, and characterization of the T. fusca enzyme, Lam81A. The Carbohydrate …
Site-specific characterization of the N-linked glycans of murine prion protein by high-performance liquid chromatography/electrospray mass spectrometry and exoglycosidase digestions.
Stimson E, Hope J, Chong A, Burlingame AL. Stimson E, et al. Biochemistry. 1999 Apr 13;38(15):4885-95. doi: 10.1021/bi982330q. Biochemistry. 1999. PMID: 10200178
The identification of the glycosylated peptide spanning Asn-197 was not reported. Recent technical advances in electrospray mass spectrometry now provide the sensitivity to detect low femtomole quantities of glycopeptides with >5000 mass resolution and 30 ppm mass measu …
The identification of the glycosylated peptide spanning Asn-197 was not reported. Recent technical advances in electrospray mass spec …
Conformation of the phosphate D-alanine zwitterion in bacterial teichoic acid from nuclear magnetic resonance spectroscopy.
Garimella R, Halye JL, Harrison W, Klebba PE, Rice CV. Garimella R, et al. Biochemistry. 2009 Oct 6;48(39):9242-9. doi: 10.1021/bi900503k. Biochemistry. 2009. PMID: 19746945 Free PMC article.
Thus, the NMR-based description of teichoic acid structure resolves the contradictory models, advances the basic understanding of cell wall biochemistry, and provides possible insight into the creation of new antibiotic therapies....
Thus, the NMR-based description of teichoic acid structure resolves the contradictory models, advances the basic understanding of cel …