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PLoS One：研究揭示淫羊藿苷对SAMP6成骨细胞的作用及机制

2018-07-12 MedSci MedSci原创

SAMP6成骨细胞淫羊藿苷
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淫羊藿苷是一种活性黄酮苷，因其在抑制成骨细胞生成和促进骨生成中的作用而被广泛用于治疗骨损伤，进行重建。衰老加速小鼠SAMP6是一种可用于揭示老年性骨质疏松症的发病机制和药物活性治疗机制的小鼠模型。然而，关于淫羊藿苷对SAMP6成骨细胞的特征及调节作用知之甚少。 从SAMP6和SAMR1小鼠中分离并培养成骨细胞，并通过进行CCK-8测定，细胞计数测定，EdU染色，细胞周期分析，ALP染色和活性测量，

淫羊藿苷是一种活性黄酮苷，因其在抑制成骨细胞生成和促进骨生成中的作用而被广泛用于治疗骨损伤，进行重建。衰老加速小鼠SAMP6是一种可用于揭示老年性骨质疏松症的发病机制和药物活性治疗机制的小鼠模型。然而，关于淫羊藿苷对SAMP6成骨细胞的特征及调节作用知之甚少。

从SAMP6和SAMR1小鼠中分离并培养成骨细胞，并通过进行CCK-8测定，细胞计数测定，EdU染色，细胞周期分析，ALP染色和活性测量，茜素红染色来对比细胞的增殖，迁移和分化。RT-qPCR分析测量成骨细胞标志物的水平，包括RUNX2，Colla1和Oc。为了评估淫羊藿苷对BMP-2诱导的成骨细胞分化的影响，在BMP-2处理后，通过RT-qPCR和半定量Western印迹分析成骨细胞标志物的表达。通过RT-qPCR测量淫羊藿苷对结缔组织生长因子（CTGF）的作用。靶向CTGF mRNA的shRNA用于敲低其在成骨细胞中的表达水平。

结果显示，与SAMR1成骨细胞相比，SAMP6成骨细胞发育和分化活性降低，表明其在与年龄相关疾病中发挥作用。此外，与SAMR1成骨细胞相比，SAMP6成骨细胞CTGF呈上调趋势。通过下调CTGF的表达淫羊藿苷可增强BMP-2诱导的成骨细胞分化。通过下调CTGF，淫羊藿苷处理上调磷酸化-Smad1/5/8，表明其对BMP信号传导通路的激活作用。

综上所述，该研究结果表明，成骨细胞的发育和功能下降可能导致与年龄相关的疾病。淫羊藿苷通过下调CTGF增强BMP-2介导的成骨细胞的成骨作用。

原始出处：

Xu B, Wang X, et al., Flavonoid compound icariin enhances BMP-2 induced differentiation and signalling by targeting to connective tissue growth factor (CTGF) in SAMP6 osteoblasts. PLoS One. 2018 Jul 10;13(7):e0200367. doi: 10.1371/journal.pone.0200367. eCollection 2018.

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    2019-01-01 119337457

    #Plos one#

    0

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    2018-07-25 tidiq

    #淫羊藿苷#

    0

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    2018-07-14 柳叶一刀

    #成骨#

    0

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    2018-07-14 jj000004

    #骨细胞#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1790240, encodeId=2bd11e90240b1, content=<a href='/topic/show?id=6e4814388e2' target=_blank style='color:#2F92EE;'>#Plos one#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14388, encryptionId=6e4814388e2, topicName=Plos one)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fda6311, createdName=119337457, createdTime=Tue Jan 01 07:16:00 CST 2019, time=2019-01-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1951714, encodeId=40dc1951e14da, content=<a href='/topic/show?id=2abb6606e9e' target=_blank style='color:#2F92EE;'>#淫羊藿苷#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66067, encryptionId=2abb6606e9e, topicName=淫羊藿苷)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3e9205, createdName=tidiq, createdTime=Wed Jul 25 10:16:00 CST 2018, time=2018-07-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351765, encodeId=ec361351e6508, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1495060, encodeId=b4bc149506099, content=<a href='/topic/show?id=3c0b10182102' target=_blank style='color:#2F92EE;'>#骨细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101821, encryptionId=3c0b10182102, topicName=骨细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=723c9147696, createdName=jj000004, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1510573, encodeId=3bd515105e396, content=<a href='/topic/show?id=7400660665b' target=_blank style='color:#2F92EE;'>#淫羊藿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66066, encryptionId=7400660665b, topicName=淫羊藿)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7e0810213212, createdName=wenjie, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=331088, encodeId=518a3310889c, content=一起学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/TDiaAWyicHgedzX3wKo3qcxudVJib7AfibfvNeufHRF2jG3Bw8MmZyWjIhtBV1mpJI7Sy9n7RcBKK5t3CuOKuOFxkg/0, createdBy=dd702206933, createdName=wzb521zf, createdTime=Thu Jul 12 14:14:18 CST 2018, time=2018-07-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=331084, encodeId=fe1733108410, content=学习了.获益匪浅.感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/02/27/c53a212859edcad4f60a54feffff50bb.jpg, createdBy=67cf1525321, createdName=13718711b3m, createdTime=Thu Jul 12 13:50:36 CST 2018, time=2018-07-12, status=1, ipAttribution=)]
    2018-07-14 wenjie

    #淫羊藿#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1790240, encodeId=2bd11e90240b1, content=<a href='/topic/show?id=6e4814388e2' target=_blank style='color:#2F92EE;'>#Plos one#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14388, encryptionId=6e4814388e2, topicName=Plos one)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fda6311, createdName=119337457, createdTime=Tue Jan 01 07:16:00 CST 2019, time=2019-01-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1951714, encodeId=40dc1951e14da, content=<a href='/topic/show?id=2abb6606e9e' target=_blank style='color:#2F92EE;'>#淫羊藿苷#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66067, encryptionId=2abb6606e9e, topicName=淫羊藿苷)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3e9205, createdName=tidiq, createdTime=Wed Jul 25 10:16:00 CST 2018, time=2018-07-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351765, encodeId=ec361351e6508, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1495060, encodeId=b4bc149506099, content=<a href='/topic/show?id=3c0b10182102' target=_blank style='color:#2F92EE;'>#骨细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101821, encryptionId=3c0b10182102, topicName=骨细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=723c9147696, createdName=jj000004, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1510573, encodeId=3bd515105e396, content=<a href='/topic/show?id=7400660665b' target=_blank style='color:#2F92EE;'>#淫羊藿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66066, encryptionId=7400660665b, topicName=淫羊藿)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7e0810213212, createdName=wenjie, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=331088, encodeId=518a3310889c, content=一起学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/TDiaAWyicHgedzX3wKo3qcxudVJib7AfibfvNeufHRF2jG3Bw8MmZyWjIhtBV1mpJI7Sy9n7RcBKK5t3CuOKuOFxkg/0, createdBy=dd702206933, createdName=wzb521zf, createdTime=Thu Jul 12 14:14:18 CST 2018, time=2018-07-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=331084, encodeId=fe1733108410, content=学习了.获益匪浅.感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/02/27/c53a212859edcad4f60a54feffff50bb.jpg, createdBy=67cf1525321, createdName=13718711b3m, createdTime=Thu Jul 12 13:50:36 CST 2018, time=2018-07-12, status=1, ipAttribution=)]
    2018-07-12 wzb521zf

    一起学习学习

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1790240, encodeId=2bd11e90240b1, content=<a href='/topic/show?id=6e4814388e2' target=_blank style='color:#2F92EE;'>#Plos one#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14388, encryptionId=6e4814388e2, topicName=Plos one)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fda6311, createdName=119337457, createdTime=Tue Jan 01 07:16:00 CST 2019, time=2019-01-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1951714, encodeId=40dc1951e14da, content=<a href='/topic/show?id=2abb6606e9e' target=_blank style='color:#2F92EE;'>#淫羊藿苷#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66067, encryptionId=2abb6606e9e, topicName=淫羊藿苷)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=b3e9205, createdName=tidiq, createdTime=Wed Jul 25 10:16:00 CST 2018, time=2018-07-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351765, encodeId=ec361351e6508, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1495060, encodeId=b4bc149506099, content=<a href='/topic/show?id=3c0b10182102' target=_blank style='color:#2F92EE;'>#骨细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101821, encryptionId=3c0b10182102, topicName=骨细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=723c9147696, createdName=jj000004, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1510573, encodeId=3bd515105e396, content=<a href='/topic/show?id=7400660665b' target=_blank style='color:#2F92EE;'>#淫羊藿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66066, encryptionId=7400660665b, topicName=淫羊藿)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7e0810213212, createdName=wenjie, createdTime=Sat Jul 14 04:16:00 CST 2018, time=2018-07-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=331088, encodeId=518a3310889c, content=一起学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/TDiaAWyicHgedzX3wKo3qcxudVJib7AfibfvNeufHRF2jG3Bw8MmZyWjIhtBV1mpJI7Sy9n7RcBKK5t3CuOKuOFxkg/0, createdBy=dd702206933, createdName=wzb521zf, createdTime=Thu Jul 12 14:14:18 CST 2018, time=2018-07-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=331084, encodeId=fe1733108410, content=学习了.获益匪浅.感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/02/27/c53a212859edcad4f60a54feffff50bb.jpg, createdBy=67cf1525321, createdName=13718711b3m, createdTime=Thu Jul 12 13:50:36 CST 2018, time=2018-07-12, status=1, ipAttribution=)]
    2018-07-12 13718711b3m

    学习了.获益匪浅.感谢分享

    0