J Mater Sci Mater Med:体外评估多种口腔生物膜在抗菌涂层钛表面的表现

2019-01-26 MedSci MedSci原创

种植体周围炎是一种感染性疾病,影响牙种植体周围的软组织和硬组织,其患病率正在显著增加。抗菌策略的开发,例如钛抗菌涂层表面,可能是预防种植体周围炎的发生和发展的有希望的策略。本研究旨在量化生物膜粘附和细菌在有或没有抗菌表面处理钛盘上的生存能力。使用五种细菌菌株建立多种口腔生物膜。所选物种代表初始(口腔链球菌和粘性放线菌),早期(Veillonella parvula),中期(Fusobacteriu

种植体周围炎是一种感染性疾病,影响牙种植体周围的软组织和硬组织,其患病率正在显著增加。抗菌策略的开发,例如钛抗菌涂层表面,可能是预防种植体周围炎的发生和发展的有希望的策略。本研究旨在量化生物膜粘附和细菌在有或没有抗菌表面处理钛盘上的生存能力。

使用五种细菌菌株建立多种口腔生物膜。所选物种代表初始(口腔链球菌和粘性放线菌),早期(Veillonella parvula),中期(Fusobacterium nucleatum)和晚期(牙龈卟啉单胞菌)定殖者。将细菌依次接种在七种不同类型的钛表面上,结合不同的粗糙度水平和抗菌涂层:银纳米颗粒和TESPSA硅烷化。使用扫描电子显微镜,共聚焦显微镜和实时PCR分析每个表面上的生物膜形成,细胞活力和细菌定量。可以观察到具有不同细菌形态的钛表面上的生物膜形成。

结果显示,与所有表面相比,TESPSA能够显著降低细胞活力(p <0.05)。与其相应的未涂覆表面相比,钛表面上的银沉积在生物膜粘附和细胞活力方面没有显示出改善的结果。细菌生物膜的总量在各组之间没有显著差异(p> 0.05)。 TESPSA能够降低生物膜粘附和细胞活力。然而,钛表面上的银沉积似乎不会赋予这些抗菌性能。

原始出处:

Vilarrasa J, Delgado LM, et al., In vitro evaluation of a multispecies oral biofilm over antibacterial coated titanium surfaces. J Mater Sci Mater Med. 2018 Nov 3;29(11):164. doi: 10.1007/s10856-018-6168-8.

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