J Mater Sci Mater Med:混合纳米羟基磷灰石/石墨烯纳米带复合材料可有效诱导成骨

2019-06-30 不详 网络

碳纳米管结合了高弯曲和机械强度,这对于许多结构和生物医学来说是有利的。最近,已经研究了一些基于碳纳米结构和纳米羟基磷灰石(nHAp)的生物材料作为骨替代物以改善再生。本研究旨在通过在不同纳米复合材料表面上培养的间充质干细胞获得一些RNA转录物(参与成骨细胞分化过程)的表达。首先使用化学气相沉积生长多壁碳纳米管(MWCNT),然后使用化学和氧等离子体处理进行剥离以获得石墨烯纳米带(GNR)。使用湿法

碳纳米管结合了高弯曲和机械强度,这对于许多结构和生物医学来说是有利的。最近,已经研究了一些基于碳纳米结构和纳米羟基磷灰石(nHAp)的生物材料作为骨替代物以改善再生。本研究旨在通过在不同纳米复合材料表面上培养的间充质干细胞获得一些RNA转录物(参与成骨细胞分化过程)的表达。

首先使用化学气相沉积生长多壁碳纳米管(MWCNT),然后使用化学和氧等离子体处理进行剥离以获得石墨烯纳米带(GNR)。使用湿法在不同量的GNR(1.0,2.0和3.0wt%)的超声辐射辅助下制备混合复合物nHAp/GNR。在细胞培养物中测试了五组。第1组:合成的nHAp;第2组:合成的GNR;第3组:nHAp和GNR的1.0%;第4组:nHAp和2.0%的GNR和第5组:nHAp和3.0%的GNR。进行实时逆转录聚合酶链反应,并将所有数据提交至Kruskal Wallis和Dunn测试,显着性水平为5%。结果,成功地生产了具有不同比例的GNR的三种纳米复合材料。细胞培养后,成骨基因的表达在组和周期之间没有显着差异。然而,在含有3.0%GNR的组中观察到骨形态发生蛋白II(BMP II),整联蛋白结合唾液蛋白(IBSP)和Osterix最高表达。

总之,我们的混合复合材料可用于需要间充质干细胞分化为成骨细胞进行愈合的骨干预。

原始出处:

de Vasconcellos LMR, do Prado RF, et al., In vitro osteogenesis process induced by hybrid nanohydroxyapatite/graphene nanoribbons composites. J Mater Sci Mater Med. 2019 Jun 28;30(7):81. doi: 10.1007/s10856-019-6271-5.

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    2019-07-02 zhaojie88
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  6. [GetPortalCommentsPageByObjectIdResponse(id=2054543, encodeId=84962054543e5, content=<a href='/topic/show?id=517c42615ef' target=_blank style='color:#2F92EE;'>#复合材料#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42615, encryptionId=517c42615ef, topicName=复合材料)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eq2AGzrugK9ZUjU3eJxzM901ophWib0xJDrSicy49ibWl3zB5pbDto7eoR6tnzwOiaKdbq1a8PcbYFFrw/132, createdBy=6ff22500098, createdName=ms4964546379600229, createdTime=Tue Feb 25 21:43:00 CST 2020, time=2020-02-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2079953, encodeId=b0c720e995320, content=<a href='/topic/show?id=225ce7444df' target=_blank style='color:#2F92EE;'>#纳米羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77444, encryptionId=225ce7444df, topicName=纳米羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e524260, createdName=dinorsor524, createdTime=Sun Jul 07 03:43:00 CST 2019, time=2019-07-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651610, encodeId=8a0916516101b, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Thu Sep 19 18:43:00 CST 2019, time=2019-09-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352175, encodeId=6dda13521e5f9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue Jul 02 12:43:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609851, encodeId=bd48160985145, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Jul 02 12:43:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622167, encodeId=5f7e162216eb5, content=<a href='/topic/show?id=f41b8000867' target=_blank style='color:#2F92EE;'>#羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Jul 02 12:43:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=368700, encodeId=b696368e0005, content=666, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/01/04/2540fb84decb81d47a7f2868070e6cbd.jpg, createdBy=ed3f2205793, createdName=百草, createdTime=Sun Jun 30 23:11:34 CST 2019, time=2019-06-30, status=1, ipAttribution=)]
  7. [GetPortalCommentsPageByObjectIdResponse(id=2054543, encodeId=84962054543e5, content=<a href='/topic/show?id=517c42615ef' target=_blank style='color:#2F92EE;'>#复合材料#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42615, encryptionId=517c42615ef, topicName=复合材料)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eq2AGzrugK9ZUjU3eJxzM901ophWib0xJDrSicy49ibWl3zB5pbDto7eoR6tnzwOiaKdbq1a8PcbYFFrw/132, createdBy=6ff22500098, createdName=ms4964546379600229, createdTime=Tue Feb 25 21:43:00 CST 2020, time=2020-02-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2079953, encodeId=b0c720e995320, content=<a href='/topic/show?id=225ce7444df' target=_blank style='color:#2F92EE;'>#纳米羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77444, encryptionId=225ce7444df, topicName=纳米羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e524260, createdName=dinorsor524, createdTime=Sun Jul 07 03:43:00 CST 2019, time=2019-07-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651610, encodeId=8a0916516101b, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Thu Sep 19 18:43:00 CST 2019, time=2019-09-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352175, encodeId=6dda13521e5f9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue Jul 02 12:43:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609851, encodeId=bd48160985145, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Jul 02 12:43:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622167, encodeId=5f7e162216eb5, content=<a href='/topic/show?id=f41b8000867' target=_blank style='color:#2F92EE;'>#羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Jul 02 12:43:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=368700, encodeId=b696368e0005, content=666, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/01/04/2540fb84decb81d47a7f2868070e6cbd.jpg, createdBy=ed3f2205793, createdName=百草, createdTime=Sun Jun 30 23:11:34 CST 2019, time=2019-06-30, status=1, ipAttribution=)]
    2019-06-30 百草

    666

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