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BMP-7过表达BM-MSCs接种生物活性玻璃/明胶纳米复合材料支架可明显改善骨再生效果

2018-01-22 MedSci MedSci原创

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在这项研究中，研究人员通过将骨髓间充质干细胞（BM-MSCs）接种到生物活性玻璃/明胶纳米复合材料支架上来评估骨形态发生蛋白-7（BMP-7）在颅骨缺损大鼠模型中的骨诱导作用。在体外收获并表征后，使用含有富含肝素结合位点的BMP-7编码基因（B2BMP-7）的质粒载体接种BM-MSCs，以评估其在体内的成骨作用。将动物随机分为三组，一组接受支架（组I），BM-MSCs接种支架（组II），另一支接种

在这项研究中，研究人员通过将骨髓间充质干细胞（BM-MSCs）接种到生物活性玻璃/明胶纳米复合材料支架上来评估骨形态发生蛋白-7（BMP-7）在颅骨缺损大鼠模型中的骨诱导作用。

在体外收获并表征后，使用含有富含肝素结合位点的BMP-7编码基因（B2BMP-7）的质粒载体接种BM-MSCs，以评估其在体内的成骨作用。将动物随机分为三组，一组接受支架（组I），BM-MSCs接种支架（组II），另一支接种预处理的BM-MSCs（组III）。术后2、4和12周后处死动物，采用组织学和免疫组织化学染色分析采集的样本。

体外试验（初步筛选）的结果显示，所合成的支架是支持细胞附着和延伸的生物相容性载体。体内实验结果显示，与其他两组相比，BMP-7分泌型骨髓间充质干细胞/支架修复骨缺损的成骨效果更好。植入12周后，整个骨缺损部位可检测到完全成熟的新形成的骨，这表明细胞、支架和生长因子在组织再生过程中具有协同作用。

因此，接种预处理的BM-MSCs的生物活性玻璃支架可改善骨缺损中的骨生成，且本研究中所采用的方法或对新型组织工程载体的研发产生重要影响。

原始出处：

Kargozar S, Hashemian SJ, et al., Acceleration of bone regeneration in bioactive glass/gelatin composite scaffolds seeded with bone marrow-derived mesenchymal stem cells over-expressing bone morphogenetic protein-7. Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:688-698. doi: 10.1016/j.msec.2017.02.097. Epub 2017 Feb 24.

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    2018-07-12 ms4964546379600229

    #复合材料#

    0

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    2018-11-04 lq1767

    #过表达#

    0

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createdTime=Fri Jan 26 07:38:26 CST 2018, time=2018-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406695, encodeId=6b2914066956c, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Wed Jan 24 13:33:00 CST 2018, time=2018-01-24, status=1, ipAttribution=)]
    2018-03-31 cooco

    #纳米复合材料#

    0

  4. 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    2018-02-06 小树熊

    学习了谢谢

    0

  5. 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createdTime=Fri Jan 26 07:38:26 CST 2018, time=2018-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406695, encodeId=6b2914066956c, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Wed Jan 24 13:33:00 CST 2018, time=2018-01-24, status=1, ipAttribution=)]
    2018-09-11 fyxzlh

    #生物活性玻璃#

    0

  6. 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createdTime=Fri Jan 26 07:38:26 CST 2018, time=2018-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406695, encodeId=6b2914066956c, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Wed Jan 24 13:33:00 CST 2018, time=2018-01-24, status=1, ipAttribution=)]
    2018-01-31 小树熊

    学习了.谢谢

    0

  7. 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topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRvvXNvY0kOJqn4liar3Q6V6yPuEIawCVUyP3jVADh5icIz0ibrsxia6fKAqKsDr33jPWrjqM3D7wcRNu/0, createdBy=5a881701729, createdName=小树熊, createdTime=Tue Feb 06 08:05:07 CST 2018, time=2018-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2005172, encodeId=d7d120051e269, content=<a href='/topic/show?id=af6e6938483' target=_blank style='color:#2F92EE;'>#生物活性玻璃#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69384, encryptionId=af6e6938483, topicName=生物活性玻璃)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c9df476, createdName=fyxzlh, createdTime=Tue Sep 11 21:33:00 CST 2018, time=2018-09-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=283673, encodeId=e39a2836e37b, content=学习了.谢谢, 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createdTime=Fri Jan 26 07:38:26 CST 2018, time=2018-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406695, encodeId=6b2914066956c, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Wed Jan 24 13:33:00 CST 2018, time=2018-01-24, status=1, ipAttribution=)]
    2018-08-04 HNYYM

    #BMP#

    0

  8. 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createdTime=Fri Jan 26 07:38:26 CST 2018, time=2018-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406695, encodeId=6b2914066956c, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Wed Jan 24 13:33:00 CST 2018, time=2018-01-24, status=1, ipAttribution=)]
    2018-01-27 小树熊

    学习了.谢谢

    0

  9. 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createdTime=Fri Jan 26 07:38:26 CST 2018, time=2018-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406695, encodeId=6b2914066956c, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Wed Jan 24 13:33:00 CST 2018, time=2018-01-24, status=1, ipAttribution=)]
    2018-01-26 小树熊

    学习了.很好的谢谢

    0

  10. 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topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRvvXNvY0kOJqn4liar3Q6V6yPuEIawCVUyP3jVADh5icIz0ibrsxia6fKAqKsDr33jPWrjqM3D7wcRNu/0, createdBy=5a881701729, createdName=小树熊, createdTime=Tue Feb 06 08:05:07 CST 2018, time=2018-02-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2005172, encodeId=d7d120051e269, content=<a href='/topic/show?id=af6e6938483' target=_blank style='color:#2F92EE;'>#生物活性玻璃#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69384, encryptionId=af6e6938483, topicName=生物活性玻璃)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c9df476, createdName=fyxzlh, createdTime=Tue Sep 11 21:33:00 CST 2018, time=2018-09-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=283673, encodeId=e39a2836e37b, content=学习了.谢谢, 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    2018-01-24 huanbaofeng

    #MSC#

    0

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