Clin Oral Investig:唾液和牙齿生物膜暴露于1500和5000 ppm氟化物后的差异分析

2017-09-12 MedSci MedSci原创

这项随机双盲交叉试验是为了检测在每天施加3次不同浓度氟化钠(0, 1500或5000 ppm)刺激3周后唾液和累积7天的生物膜流体和干物内的氟化物变化。

这项随机双盲交叉试验是为了检测在每天施加3次不同浓度氟化钠(0, 1500或5000 ppm)刺激3周后唾液和累积7天的生物膜流体和干物内的氟化物变化。

样本进行3周的洗涤(包括1周生物膜累积),随后即刻收集12名志愿者在每次洗涤后0(背景氟化物), 10, 30和60 min的唾液和生物膜样本。将生物膜样本分为流体和干物,并通过氟电极进行微量分析。

结果显示,5000 ppm氟洗涤组的背景氟化物浓度在所有3个时间间隔内均明显高于1500 ppm组(唾液为22.3和8.1 μM, 生物膜流体为126.8和58.5 μM, 生物膜干物为10,940和4837 μmol/kg)。在全部3个时间间隔内,5000 ppm氟洗组的1小时氟化物累积要显著多于1500 ppm组,尽管唾液和氟化物干物之间没有显著性差异。

结论:有规则的使用5000 ppm氟化物进行洗涤要比1500 mmp明显上调唾液、生物膜流体和干物的背景氟化物浓度。几乎上升了3.5倍的氟化物浓度(从1500至5000 ppm)仅上调唾液、生物膜流体和干物的背景氟化物浓度2倍。

临床意义:即使含氟牙膏受到唾液的稀释,本项研究结果依旧表明,使用5000 ppm的含氟牙膏可以有效控制龋坏进展。

原始出处

Staun Larsen L, Baelum V, et al. Fluoride in saliva and dental biofilm after 1500 and 5000 ppm fluoride exposure. Clin Oral Investig. 2017 Sep 1. doi: 10.1007/s00784-017-2195-y.

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    2017-12-15 小几洁
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    2017-09-14 zhouqu_8
  5. [GetPortalCommentsPageByObjectIdResponse(id=2020228, encodeId=3f50202022889, content=<a href='/topic/show?id=1584134451c' target=_blank style='color:#2F92EE;'>#Oral#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13445, encryptionId=1584134451c, topicName=Oral)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIpeueeYB1kem1yfEZrSYKBdZBoqac9USf0RxIgRICBIyDn4licyiaiakNnobeCDVpvwqaWgwTNeLRHA/132, createdBy=bb5e2500127, createdName=小几洁, createdTime=Fri Dec 15 08:01:00 CST 2017, time=2017-12-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2050845, encodeId=fb6d2050845d2, content=<a href='/topic/show?id=95f8e042dd' target=_blank style='color:#2F92EE;'>#EST#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7042, encryptionId=95f8e042dd, topicName=EST)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e9a6357, createdName=xiangyuzhou971, createdTime=Sat Dec 30 17:01:00 CST 2017, time=2017-12-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1405098, encodeId=bc221405098f8, content=<a href='/topic/show?id=f5ef63909a3' target=_blank style='color:#2F92EE;'>#氟化物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63909, encryptionId=f5ef63909a3, topicName=氟化物)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=d4322523154, createdName=rebeccajiejie, createdTime=Thu Sep 14 08:01:00 CST 2017, time=2017-09-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1430094, encodeId=9237143009483, content=<a href='/topic/show?id=b58f6e691fc' target=_blank style='color:#2F92EE;'>#牙齿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67691, encryptionId=b58f6e691fc, topicName=牙齿)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Thu Sep 14 08:01:00 CST 2017, time=2017-09-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=243437, encodeId=935f24343e1e, content=非常好的文章.学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7LIiaRZTzJ3SEfiauCzTMPW7YvPPYLNJBXG9oh6Al1icq2VQQkIHWxqXehcicTS62YKJVBhxeth3wggw/0, createdBy=7ce01621306, createdName=天涯183, createdTime=Wed Sep 13 06:04:06 CST 2017, time=2017-09-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=243291, encodeId=a63d24329165, content=即使含氟牙膏受到唾液的稀释.本项研究结果依旧表明.使用5000ppm的含氟牙膏可以有效控制龋坏进展., beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7cJ6ejWUr2nIia8qOvAKNdLHr32Df6G8iaoaOx6pHaoiacwp26DaslxZHCGbibZhhCGven4rvmWP60GahtzgFjgCjR/0, createdBy=6b111703011, createdName=有备才能无患, createdTime=Tue Sep 12 19:07:21 CST 2017, time=2017-09-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=243273, encodeId=76b62432e345, content=学习一下, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6KcicAVC1MwuOgo3iaicb1Imgk1yIfnvr6oXzibcAFB6puAyNJXbzWhNuLqaFYBVRWjh7rghkvFLPxMAfD5ib3nXwVD/0, createdBy=bed21972958, createdName=wxl882001, createdTime=Tue Sep 12 18:38:29 CST 2017, time=2017-09-12, status=1, ipAttribution=)]
    2017-09-13 天涯183

    非常好的文章.学习了

    0

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    2017-09-12 有备才能无患

    即使含氟牙膏受到唾液的稀释.本项研究结果依旧表明.使用5000ppm的含氟牙膏可以有效控制龋坏进展.

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=2020228, encodeId=3f50202022889, content=<a href='/topic/show?id=1584134451c' target=_blank style='color:#2F92EE;'>#Oral#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13445, encryptionId=1584134451c, topicName=Oral)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIpeueeYB1kem1yfEZrSYKBdZBoqac9USf0RxIgRICBIyDn4licyiaiakNnobeCDVpvwqaWgwTNeLRHA/132, createdBy=bb5e2500127, createdName=小几洁, createdTime=Fri Dec 15 08:01:00 CST 2017, time=2017-12-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2050845, encodeId=fb6d2050845d2, content=<a href='/topic/show?id=95f8e042dd' target=_blank style='color:#2F92EE;'>#EST#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7042, encryptionId=95f8e042dd, topicName=EST)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e9a6357, createdName=xiangyuzhou971, createdTime=Sat Dec 30 17:01:00 CST 2017, time=2017-12-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1405098, encodeId=bc221405098f8, content=<a href='/topic/show?id=f5ef63909a3' target=_blank style='color:#2F92EE;'>#氟化物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63909, encryptionId=f5ef63909a3, topicName=氟化物)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=d4322523154, createdName=rebeccajiejie, createdTime=Thu Sep 14 08:01:00 CST 2017, time=2017-09-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1430094, encodeId=9237143009483, content=<a href='/topic/show?id=b58f6e691fc' target=_blank style='color:#2F92EE;'>#牙齿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67691, encryptionId=b58f6e691fc, topicName=牙齿)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Thu Sep 14 08:01:00 CST 2017, time=2017-09-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=243437, encodeId=935f24343e1e, content=非常好的文章.学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7LIiaRZTzJ3SEfiauCzTMPW7YvPPYLNJBXG9oh6Al1icq2VQQkIHWxqXehcicTS62YKJVBhxeth3wggw/0, createdBy=7ce01621306, createdName=天涯183, createdTime=Wed Sep 13 06:04:06 CST 2017, time=2017-09-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=243291, encodeId=a63d24329165, content=即使含氟牙膏受到唾液的稀释.本项研究结果依旧表明.使用5000ppm的含氟牙膏可以有效控制龋坏进展., beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7cJ6ejWUr2nIia8qOvAKNdLHr32Df6G8iaoaOx6pHaoiacwp26DaslxZHCGbibZhhCGven4rvmWP60GahtzgFjgCjR/0, createdBy=6b111703011, createdName=有备才能无患, createdTime=Tue Sep 12 19:07:21 CST 2017, time=2017-09-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=243273, encodeId=76b62432e345, content=学习一下, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6KcicAVC1MwuOgo3iaicb1Imgk1yIfnvr6oXzibcAFB6puAyNJXbzWhNuLqaFYBVRWjh7rghkvFLPxMAfD5ib3nXwVD/0, createdBy=bed21972958, createdName=wxl882001, createdTime=Tue Sep 12 18:38:29 CST 2017, time=2017-09-12, status=1, ipAttribution=)]
    2017-09-12 wxl882001

    学习一下

    0

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不同细菌刺激上皮细胞的反应不同。生物膜组成和活性可能影响上皮反应。通常用体外模型系统研究牙周炎相关细菌及其与宿主的相互作用;因此,理解影响生物膜 - 细胞相互作用的因素至关重要。本研究旨在开发体外单种和多种生物膜,并调查上皮对这些生物膜的反应。

mBio:科学家呼吁研究生物膜才可更好的对抗感染

据发表于mBio的一项新的研究显示,从菌斑中释放出来的微生物生物膜较单个的细菌更具有抵抗力,因此,需要改变我们既往所认为的感染方式。