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J Periodontol：超声洁治VS.激光治疗种植体周围炎的效果

2018-02-05 MedSci MedSci原创

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本研究旨在评估常规超声洁牙机与铒：钇铝石榴石（Er：YAG）激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响：1）牙菌斑和生物腐蚀氧化钛涂层的去除；2）表面改变诱导；和3）对成骨细胞的生物相容性。将龈下菌斑涂覆在适度粗糙表面的钛片，然后乙醇固定，并且以非接触模式用超声波洁牙机（金属尖端）或Er：YAG激光（20.3或38.2J/cm 2）处理。荧光检测残留菌斑。通过扫描电子显微镜评估钛片表面形态

本研究旨在评估常规超声洁牙机与铒：钇铝石榴石（Er：YAG）激光对受种植体周围炎患者龈下菌斑污染的钛表面的影响：1）牙菌斑和生物腐蚀氧化钛涂层的去除；2）表面改变诱导；和3）对成骨细胞的生物相容性。

将龈下菌斑涂覆在适度粗糙表面的钛片，然后乙醇固定，并且以非接触模式用超声波洁牙机（金属尖端）或Er：YAG激光（20.3或38.2J/cm 2）处理。荧光检测残留菌斑。通过扫描电子显微镜评估钛片表面形态。通过碘化丙锭/DNA染色试验分析Saos-2成骨细胞在新的和处理过的钛片表面的生存能力、附着、增殖和分化；共聚焦显微镜分析细胞骨架、Ki67、骨桥蛋白和碱性磷酸酶的表达以及矿化结节的形成。

两种方法都能有效地清洁钛片表面，超声波洁牙机可使斑块面积减少到11.7%，Er：YAG激光（38.2 J/cm2）可减少到≤0.03％。超声处理的钛片表面表现出明显的变化，二氧化钛（TiO2）层的不完全去除和少量斑块残留，而Er：YAG激光（38.2J/cm 2）可完全去除斑块和TiO2层，开放钛表面微观结构。两种处理都保持Saos-2成骨细胞表面良好的生物相容性。气-水冷却保持钛片温度低于47℃的临界阈值。

综上所述，这项研究表明，具有金属尖端的超声波洁牙机在去除阳极氧化钛片上的斑块和TiO2层方面不如高能量Er：YAG激光的效果好，虽然两种方法似乎能够恢复处理过的钛表面足够的骨导电率。

原始出处：

Giannelli M, Bani D, et al., Effects of an Erbium:Yttrium-Aluminum-Garnet Laser and Ultrasonic Scaler on Titanium Dioxide-Coated Titanium Surfaces Contaminated With Subgingival Plaque: An In Vitro Study to Assess Post-Treatment Biocompatibility With Osteogenic Cells. J Periodontol. 2017 Nov;88(11):1211-1220. doi: 10.1902/jop.2017.170195. Epub 2017 Jul 10.

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    2019-01-15 hyf028

    #光治疗#

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    2018-07-27 feather89

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    #激光#

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    2018-02-07 ms6279672939590805

    #种植体#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=2033284, encodeId=4032203328446, content=<a href='/topic/show?id=692328816d7' target=_blank style='color:#2F92EE;'>#光治疗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=28816, encryptionId=692328816d7, topicName=光治疗)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7d40148, createdName=hyf028, createdTime=Tue Jan 15 20:35:00 CST 2019, time=2019-01-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727047, encodeId=82af1e270479f, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Fri Jul 27 00:35:00 CST 2018, time=2018-07-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701912, encodeId=01cb1e0191270, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Wed Jul 25 06:35:00 CST 2018, time=2018-07-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1279701, encodeId=529a12e97012c, content=<a href='/topic/show?id=265c66e50ea' target=_blank style='color:#2F92EE;'>#激光#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66750, encryptionId=265c66e50ea, topicName=激光)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4fd6156, createdName=oliver169, createdTime=Wed Feb 07 06:35:00 CST 2018, time=2018-02-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343762, encodeId=be3e1343e62c8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Wed Feb 07 06:35:00 CST 2018, time=2018-02-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1434716, encodeId=93fc1434e1628, content=<a href='/topic/show?id=817f66e686a' target=_blank style='color:#2F92EE;'>#激光治疗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=66768, encryptionId=817f66e686a, topicName=激光治疗)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Wed Feb 07 06:35:00 CST 2018, time=2018-02-07, status=1, ipAttribution=)]
    2018-02-07 zhouqu_8

    #激光治疗#

    0