INT J LAB HEMATOL:外周血涂片评估中的实时远程数字显微镜检查----观察者内部的一致性和经验

2019-02-01 MedSci MedSci原创

外周血涂片(PBS)审查是一项常规实验室检查,需要病理学家在发现异常指标,非典型细胞或发现关键问题时进行解释。当现场病理学家不可用时,实时远程数字显微镜(DM)可能有助于快速检查。在此,研究人员使用VisionTek M6机器人DM和TeamViewer成像软件评估PBS评估的观察者与光学显微镜(LM)和DM的一致性。 <span style="font-family:宋体;mso-asci

外周血涂片(PBS)审查是一项常规实验室检查,需要病理学家在发现异常指标,非典型细胞或发现关键问题时进行解释。当现场病理学家不可用时,实时远程数字显微镜(DM)可能有助于快速检查。在此,研究人员使用VisionTek M6机器人DMTeamViewer成像软件评估PBS评估的观察者与光学显微镜(LM)和DM的一致性。

由五名评价者评估了37个去识别标记的PBS载玻片。载玻片在异地实验室的VisionTek M6机器人显微镜上加载,并通过TeamViewer软件远程评估。评审员记录了解释,所需的时间(以分钟为单位),成像质量(0-3分)和解释置信度(0-3分)。此外,还记录了与DM评估相关的其他相关信息。随后在洗出间隔后使用LM评估载玻片。在DMLM之间比较观察者的印象,数字幻灯片质量,解释分钟和解释置信度的结果。

研究发现,LMDM之间的观察者内部一致性为93%,在每种评价模式下的135次评估中分别有9种不一致的解释。虽然审查人在DM模式下(5分钟/幻灯片)比LM模式(2.5分钟/幻灯片)花费更多时间,但评论者认为DM提供了足够的图像质量,并且评论者对幻灯片解释的置信水平在DM之间具有可比性(2.6 / 3 )和LM2.8 / 3)。

DMLM之间观察者内部一致性和可比较的解释置信度较高。DM可以作为非现场病理学家对PBS的评估的有用方法。

原始出处:

Diana M. Haninger Mehdi Nassiri Elizabeth D. Settembre. Live remote digital microscopy in peripheral blood smear evaluation: Intraobserver concordance and experience

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    2019-02-11 zhanfl
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    2019-07-03 tulenzi
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    2019-02-03 fengyi812
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