J Photochem Photobiol B:新型G-AgNPs或可有效治疗伤口感染

2017-11-28 MedSci MedSci原创

近年来,银纳米粒子(AgNPs)由于其重要的物理化学和抗菌性能,在药物、生物医学和药物研究领域引起了研究人员的广泛关注。芦荟叶汁提取物(LSE)含有很多活性成分,具有较多医学应用(包括伤口愈合)。本研究中,研究人员使用LSE作为还原剂、稳定剂和封端剂在 “绿色合成”(G-AgNPs)中制备AgNPs。本研究旨在准备、表征和评估G-AgNPs对人类致病菌的杀灭潜力,以用于治疗感染伤口。将硝酸银溶液和

近年来,银纳米粒子(AgNPs)由于其重要的物理化学和抗菌性能,在药物、生物医学和药物研究领域引起了研究人员的广泛关注。芦荟叶汁提取物(LSE)含有很多活性成分,具有较多医学应用(包括伤口愈合)。

本研究中,研究人员使用LSE作为还原剂、稳定剂和封端剂在 “绿色合成”(G-AgNPs)中制备AgNPs。本研究旨在准备、表征和评估G-AgNPs对人类致病菌的杀灭潜力,以用于治疗感染伤口。将硝酸银溶液和LSE的混合物暴露于直射的阳光下,溶液从无色至红褐色的快速颜色变化,表明形成G-AgNP。

探究G-AgNP的理化性质和表面化学特性,分别使用使用单粒子电感耦合等离子体质谱、高分辨透射电子显微镜和傅里叶变换红外光谱和X射线衍射等进行探究。结果显示,G-AgNP粒径为38±2nm,表面平滑光整,表面存在LSE。

抗菌方面显示,G-AgNPs对铜绿假单胞菌和金黄色葡萄球菌都具有良好的抗菌活性。流式细胞术研究显示,G-AgNPs通过细胞膜损伤处理的死细胞百分比增加,并且通过共焦激光扫描显微镜进一步证实。

因此,本研究揭示了新型G-AgNPs对革兰氏阴性和革兰氏阳性菌株均显示出有效的抗菌特性,在治疗病原体感染伤口中具有巨大潜力。

原始出处:

Kumar SSD, Houreld NN, Kroukamp EM, Abrahamse H. Cellular imaging and bactericidal mechanism of green-synthesized silver nanoparticles against human pathogenic bacteria. J Photochem Photobiol B. 2017 Nov 3;178:259-269. doi: 10.1016/j.jphotobiol.2017.11.001.

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    2018-09-16 liuxiaona
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    2018-07-25 sunylz
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    2017-12-07 虈亣靌

    不错的.学习了!谢谢分享!

    0

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    2017-11-28 1dd8c52fm63(暂无匿称)

    了解了解.继续关注

    0