Allergy:花生过敏的诊断方法比较!

2017-07-09 xing.T MedSci原创

由此可见,BAT显示了显著升高的CDsens相比于HR和被动HR。嗜碱性粒细胞检验的诊断性能差异不明显。不过,BAT可以诊断低嗜碱性粒细胞数的受试者,与HR相反。

过敏可以用嗜碱性粒细胞检查来进行确诊。有几种方法可以检测嗜碱性粒细胞的活化。近日,过敏性疾病领域权威杂志Allergy上发表了一篇研究文章,该研究旨在比较嗜碱性粒细胞活化试验(BAT)、组胺释放试验(HR)和被动致敏的组胺释放试验(被动HR)在花生过敏诊断中的效能。研究人员对十一例花生过敏者和十四例非过敏性受试者进行BAT、HR和被动HR检测。血液与花生提取物或抗IgE孵育,检测步骤如下:BAT-流式细胞仪检测CD63表达上调,后细胞释放组胺;HR-采用玻璃纤维定量荧光法定量组胺释放;被动HR-IgE剥离捐赠者的嗜碱性粒细胞与参与者的血清孵育并且组胺释放量化为HR。CDsens,一个衡量嗜碱性粒细胞过敏原敏感性的指标,显著高于BAT(80.1±17.4),相比于HR(23.4±10.31)和被动HR(11.1±2)。BAT、HR和被动HR的临床敏感性分别为100%、100%和82%,特异性分别为100%、100%和100%,分别排除不确定的结果。BAT确诊了11名过敏患者中的11人,HR确诊了10人和被动HR确诊可9人。同样地,BAT识别了14个非过敏者中的12个,HR识别可10人和被

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    2017-10-07 gous
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    2017-07-16 松鼠上树

    学习了,谢谢分享

    0

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    2017-07-11 膀胱癌
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