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Pak J Pharm Sci：Ad-BMP-2-IRES-HIF-1α转染的EPCs具有较强的血管生成和成骨能力

2017-12-02 MedSci MedSci原创

Ad-BMP-2-IRES-HIF-1α成血管成骨
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本研究旨在探究将Ad-BMP-2-IRES-HIF-1αmu转染EPCs，然后移植入股骨头坏死区，检测坏死区的成骨和成骨作用情况。首先将Ad-BMP-2-IRES-HIF-1α转染EPCs，然后移植于股骨头缺血坏死部位（ANFH），继而检测Ad-BMP-2-IRES-HIF-1α对坏死部位血管生成和成骨能力。采用密度梯度离心法获得兔骨髓单核细胞，然后使用M199培养基诱导分化为EPCs，根据细胞形

本研究旨在探究将Ad-BMP-2-IRES-HIF-1αmu转染EPCs，然后移植入股骨头坏死区，检测坏死区的成骨和成骨作用情况。

首先将Ad-BMP-2-IRES-HIF-1α转染EPCs，然后移植于股骨头缺血坏死部位（ANFH），继而检测Ad-BMP-2-IRES-HIF-1α对坏死部位血管生成和成骨能力。采用密度梯度离心法获得兔骨髓单核细胞，然后使用M199培养基诱导分化为EPCs，根据细胞形态学、特异性表面标记和摄取能力鉴定EPCs。最后将Ad-BMP-2-IRES-HIF-1α转染EPCs移植到ANFH部分。术后2周、4周实施安乐死，检测坏死部位的血管生成和成骨指标。

结果显示，A组产生的血管数多于B组和C组（P <0.05），B组与C组比较差异有统计学意义（P <0.05）。组织学检测和BMP-2免疫组化结果显示，A组与B组，A组和C组比较差异有统计学意义（P <0.05）。 B组与C组比较差异无统计学意义（P> 0.05）。

综上所述，本实验表明，Ad-BMP-2-IRES-HIF-1α转染的EPCs具有较强的血管生成和成骨能力。

原始出处：

Cui F, Wang X, et al., Detection of AD-BMP-2-IRES-HIF-1α MU on local promoting angiogenic and osteogenic capacity of necrosis area. Pak J Pharm Sci. 2017 Sep;30(5(Supplementary)):2013-2019.

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    2018-03-29 jj000001

    #Pharm#

    0

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    2018-02-10 12498f17m73暂无昵称

    #BMP-2#

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    2018-03-10 HNYYM

    #BMP#

    0

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    2018-04-07 Tamikia

    #PCS#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1787398, encodeId=2be41e873985c, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8942242, createdName=jj000001, createdTime=Thu Mar 29 13:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642645, encodeId=b3c11642645be, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Feb 10 16:11:00 CST 2018, time=2018-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634014, encodeId=d2ef163401450, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Sat Mar 10 22:11:00 CST 2018, time=2018-03-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1685599, encodeId=c4a416855996c, content=<a href='/topic/show?id=a340138e48a' target=_blank style='color:#2F92EE;'>#PCS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13874, encryptionId=a340138e48a, topicName=PCS)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=173d28230918, createdName=Tamikia, createdTime=Sat Apr 07 14:11:00 CST 2018, time=2018-04-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351637, encodeId=eda3135163e90, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522146, encodeId=34ab152214628, content=<a href='/topic/show?id=1b34881e59' target=_blank style='color:#2F92EE;'>#HIF-1α#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8817, encryptionId=1b34881e59, topicName=HIF-1α)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=847f11414102, createdName=changfy, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1556027, encodeId=04b1155602ebe, content=<a href='/topic/show?id=b3e98813c3' target=_blank style='color:#2F92EE;'>#HIF#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8813, encryptionId=b3e98813c3, topicName=HIF)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=293014689254, createdName=wrj0126, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266229, encodeId=a15b266229ec, content=学习了.获益匪浅!感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/07/17/0ed05bbc4eb124766d2a39104932ec4e.jpg, createdBy=5afd2091871, createdName=情途末路, createdTime=Sat Dec 02 17:21:28 CST 2017, time=2017-12-02, status=1, ipAttribution=)]
    2017-12-04 zhaojie88

    #成骨#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1787398, encodeId=2be41e873985c, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8942242, createdName=jj000001, createdTime=Thu Mar 29 13:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642645, encodeId=b3c11642645be, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Feb 10 16:11:00 CST 2018, time=2018-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634014, encodeId=d2ef163401450, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Sat Mar 10 22:11:00 CST 2018, time=2018-03-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1685599, encodeId=c4a416855996c, content=<a href='/topic/show?id=a340138e48a' target=_blank style='color:#2F92EE;'>#PCS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13874, encryptionId=a340138e48a, topicName=PCS)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=173d28230918, createdName=Tamikia, createdTime=Sat Apr 07 14:11:00 CST 2018, time=2018-04-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351637, encodeId=eda3135163e90, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522146, encodeId=34ab152214628, content=<a href='/topic/show?id=1b34881e59' target=_blank style='color:#2F92EE;'>#HIF-1α#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8817, encryptionId=1b34881e59, topicName=HIF-1α)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=847f11414102, createdName=changfy, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1556027, encodeId=04b1155602ebe, content=<a href='/topic/show?id=b3e98813c3' target=_blank style='color:#2F92EE;'>#HIF#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8813, encryptionId=b3e98813c3, topicName=HIF)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=293014689254, createdName=wrj0126, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266229, encodeId=a15b266229ec, content=学习了.获益匪浅!感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/07/17/0ed05bbc4eb124766d2a39104932ec4e.jpg, createdBy=5afd2091871, createdName=情途末路, createdTime=Sat Dec 02 17:21:28 CST 2017, time=2017-12-02, status=1, ipAttribution=)]
    2017-12-04 changfy

    #HIF-1α#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1787398, encodeId=2be41e873985c, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8942242, createdName=jj000001, createdTime=Thu Mar 29 13:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642645, encodeId=b3c11642645be, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Feb 10 16:11:00 CST 2018, time=2018-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634014, encodeId=d2ef163401450, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Sat Mar 10 22:11:00 CST 2018, time=2018-03-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1685599, encodeId=c4a416855996c, content=<a href='/topic/show?id=a340138e48a' target=_blank style='color:#2F92EE;'>#PCS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13874, encryptionId=a340138e48a, topicName=PCS)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=173d28230918, createdName=Tamikia, createdTime=Sat Apr 07 14:11:00 CST 2018, time=2018-04-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351637, encodeId=eda3135163e90, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522146, encodeId=34ab152214628, content=<a href='/topic/show?id=1b34881e59' target=_blank style='color:#2F92EE;'>#HIF-1α#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8817, encryptionId=1b34881e59, topicName=HIF-1α)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=847f11414102, createdName=changfy, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1556027, encodeId=04b1155602ebe, content=<a href='/topic/show?id=b3e98813c3' target=_blank style='color:#2F92EE;'>#HIF#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8813, encryptionId=b3e98813c3, topicName=HIF)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=293014689254, createdName=wrj0126, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266229, encodeId=a15b266229ec, content=学习了.获益匪浅!感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/07/17/0ed05bbc4eb124766d2a39104932ec4e.jpg, createdBy=5afd2091871, createdName=情途末路, createdTime=Sat Dec 02 17:21:28 CST 2017, time=2017-12-02, status=1, ipAttribution=)]
    2017-12-04 wrj0126

    #HIF#

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1787398, encodeId=2be41e873985c, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8942242, createdName=jj000001, createdTime=Thu Mar 29 13:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642645, encodeId=b3c11642645be, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Feb 10 16:11:00 CST 2018, time=2018-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634014, encodeId=d2ef163401450, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Sat Mar 10 22:11:00 CST 2018, time=2018-03-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1685599, encodeId=c4a416855996c, content=<a href='/topic/show?id=a340138e48a' target=_blank style='color:#2F92EE;'>#PCS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13874, encryptionId=a340138e48a, topicName=PCS)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=173d28230918, createdName=Tamikia, createdTime=Sat Apr 07 14:11:00 CST 2018, time=2018-04-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351637, encodeId=eda3135163e90, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1522146, encodeId=34ab152214628, content=<a href='/topic/show?id=1b34881e59' target=_blank style='color:#2F92EE;'>#HIF-1α#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8817, encryptionId=1b34881e59, topicName=HIF-1α)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=847f11414102, createdName=changfy, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1556027, encodeId=04b1155602ebe, content=<a href='/topic/show?id=b3e98813c3' target=_blank style='color:#2F92EE;'>#HIF#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8813, encryptionId=b3e98813c3, topicName=HIF)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=293014689254, createdName=wrj0126, createdTime=Mon Dec 04 08:11:00 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266229, encodeId=a15b266229ec, content=学习了.获益匪浅!感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/07/17/0ed05bbc4eb124766d2a39104932ec4e.jpg, createdBy=5afd2091871, createdName=情途末路, createdTime=Sat Dec 02 17:21:28 CST 2017, time=2017-12-02, status=1, ipAttribution=)]
    2017-12-02 情途末路

    学习了.获益匪浅!感谢分享

    0