Sci Rep:研究确定卵巢癌中差异表达的LncRNA

2017-09-08 MedSci MedSci原创

癌相关成纤维细胞(CAFs)与卵巢癌预后较差相关。与肿瘤细胞不同,CAFsDNA突变很罕见,增加了其他调节基因(如长非编码RNAs的可能性(lncRNAs))表达机制的可能性。本研究旨在确定促进CAFs的肿瘤促进表型的lncRNA。研究共纳入了67个卵巢CAF标本和10个正常卵巢细胞(NOF)样本进行RNA表达分析,以识别差异表达的lncRNA,并构建功能网络以预测参与转移CAF特异性lncRNA

癌相关成纤维细胞(CAFs)与卵巢癌预后较差相关。与肿瘤细胞不同,CAFsDNA突变很罕见,增加了其他调节基因(如长非编码RNAs的可能性(lncRNAs))表达机制的可能性。本研究旨在确定促进CAFs的肿瘤促进表型的lncRNA。

研究共纳入了67个卵巢CAF标本和10个正常卵巢细胞(NOF)样本进行RNA表达分析,以识别差异表达的lncRNA,并构建功能网络以预测参与转移CAF特异性lncRNAs。

基因表达芯片共检测出1970个可供分析的lncRNAs,其中39个特异性lncRNAs在CAF与NOFS中差异表达。使用多变量模型评估鉴别差异表达lncRNA的预测能力。已知的转录因子相互作用,转录因子基因相互作用,以及构建一个上下游特定的相互作用网络来确定多种lncRNAs可能在转移中发挥重要作用。

总之,该研究发现了卵巢癌中差异表达的lncRNAs,并可有助于预测CAF转移促进表型。

原始出处:

Fatemeh Vafaee, Emily K. Colvin, et al. Functional prediction of long non-coding RNAs in ovarian cancer-associated fibroblasts indicate a potential role in metastasis.Sci Rep. 2017; 7: 10374. Published online 2017 Sep 4. doi: 10.1038/s41598-017-10869-y

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    2017-09-10 xzw113
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    2017-09-10 yfjms

    学习了

    0

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    2017-09-09 luominglian113

    学习了.谢谢分享

    0

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    2017-09-08 184****9840

    学习了受益匪浅

    0

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卵巢癌是世界上最常见的三大妇科恶性肿瘤之一。由于治疗策略的限制,这种恶性肿瘤患者的预后仍然很差。本研究中,研究人员探究了了一种长链非编码RNA (MIR4697HG)在卵巢癌细胞生长与转移中的作用。结果表明,癌组织中MIR4697HG的转录水平较癌旁组织增加了一倍。卵巢癌细胞系中MIR4697HG的表达不同,OVCAR3 和 SKOV3细胞中表达水平最高。通过特定的shRNA敲除MIR4697HG

Int J Cancer:痛经会导致卵巢癌风险增加

痛经是一种常见的妇科疾病,指行经前后或月经期出现下腹部疼痛、坠胀,伴有腰酸或其他不适,症状严重影响生活质量者。痛经分为原发性痛经和继发性两类,原发性痛经指生殖器官无器质性病变的痛经,占痛经90%以上;继发性痛经指由盆腔器质性疾病引起的痛经。在既往的研究中,部分研究的结果表明它与女性患卵巢癌的风险相关,但并非所有的研究都如此。此外,由于缺乏动力,痛经和卵巢癌的不同组织学亚型之间的潜在变化还没有得到充

Int J Cancer:抗抑郁药或能降低卵巢上皮癌的风险

卵巢上皮癌是常见的妇科恶性肿瘤,占卵巢肿瘤的;50%-70%.由于EOC早期(I、II期)缺乏典型临床表观。76%的病人在发现时已有腹腔内种植,肝实质和/或胸腔、颅内转移(III、IV期)这是导致较高病死率的主要原因。 抗抑郁药治疗在女性中被广泛使用,主要治疗抑郁症和焦虑症,但目前很少有研究探讨抗抑郁药对妇科癌症风险的影响。

Oncotarget:老标记新功能,血清CA125浓度预测卵巢癌转移

卵巢恶性肿瘤是女性生殖器官常见的恶性肿瘤之一,对妇女生命造成严重威胁。由于卵巢的胚胎发育、组织解剖及内分泌功能较复杂,早期症状不典型,术前鉴别卵巢肿瘤的组织类型及良恶性相当困难。

J CLIN ONCOL:延长无铂间期能否为铂类部分敏感的卵巢癌患者带来生存获益?

对于无铂间期(PFI)6至12个月的卵巢癌复发患者,临床上通过引入非铂药物来延长PFI并增加患者对铂类药物的敏感性。但是由Sandro Pignata教授主导于2017年8月21日发表在JOURNAL OF CLINICAL ONCOLOGY上的MITO-8研究则发现,这种治疗策略并不使对铂类药物部分敏感的卵巢癌患者取得生存获益。

Mol Cancer:研究发现卵巢癌新的治疗靶点

越来愈多的研究表明lncRNAs在肿瘤细胞的增殖、分化和凋亡中发挥着重要的作用。研究人员使用实时荧光定量PCR(qRT-PCR)研究了上皮性卵巢癌(EOC)和正常卵巢组织中lncRNA ABHD11-AS1的表达。通过小干扰RNA(siRNA)下调ABHD11-AS1或质粒转染过表达ABHD11-AS1,检测卵巢癌细胞表型及相关分子的表达。结果显示,在卵巢癌组织中的lncRNA ABHD11-AS