J Periodontal Res:HIV感染可改变患者龈下菌斑微生物的组成

2015-06-12 MedSci MedSci原创

J Periodontal Res:HIV感染可改变患者龈下菌斑微生物的组成本研究旨在通过变性梯度凝胶电泳(DGGE)对比伴慢性牙周炎的HIV阴性个体与HIV阳性个体其龈下菌斑微生物的种类。研究共纳入32名患者:11名HIV阳性患者,21名HIV阴性患者,所有研究对象均为慢性牙周炎患者。测量每位研究对象的牙周袋深度,临床附着水平,龈上菌斑微生物以及探针出血情况。收集患者龈下菌斑微生物样品(50%

本研究旨在通过变性梯度凝胶电泳(DGGE)对比伴慢性牙周炎的HIV阴性个体与HIV阳性个体其龈下菌斑微生物的种类。


研究共纳入32名患者:11名HIV阳性患者,21名HIV阴性患者,所有研究对象均为慢性牙周炎患者。测量每位研究对象的牙周袋深度,临床附着水平,龈上菌斑微生物以及探针出血情况。收集患者龈下菌斑微生物样品(50% 牙周袋探诊深度≤ 4 mm ,50% 牙周袋探诊深度≥ 5 mm),并获得所有研究对象的全基因组扩增的DNA。 使用通用细菌引物扩增16S rRNA 基因片段,然后通过DGGE分析进行测序。

结果发现,与HIV阳性患者相比,HIV阴性患者的全口龈上菌斑较多 (p = 0.004),探针出血水平较高 (p = 0.006),探诊深度较深 (p < 0.001),临床附着丧失水平也较大(p = 0.001)。DGGE分析显示32名患者显示出81种不同的条带。从条带可以看出,伴慢性牙周炎HIV阳性的患者其龈下菌斑微生物的种类更多。此外,对菌落分析显示,HIV阳性和HIV阴性个体,其菌群成分差别较大,但HIV阳性和HIV阴性患者均发现个体间个个体内差异均较大。

总而言之,伴慢性牙周炎的HIV阳性患者其龈下菌斑微生物的种类增多。此外,HIV阳性患者与HIV阴性患者的龈下菌斑微生物的种类结构不同。

原始出处:

Ferreira DC, Gonçalves LS, Siqueira JF Jr, Carmo FL, Santos HF, Feres M, Figueiredo LC, Soares GM, Rosado AS, Dos Santos KR, Colombo AP. Subgingival bacterial community profiles in HIV-infected Brazilian adults with chronic periodontitis.J Periodontal Res.June 3, 2015. doi: 10.1111/jre.12287. 

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    2016-09-13 1e10c84am36(暂无匿称)

    好文章,还会关注

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    2016-05-23 wrj0131
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  4. [GetPortalCommentsPageByObjectIdResponse(id=125050, encodeId=cd8f12505041, content=好文章,还会关注, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Tue Sep 13 11:51:00 CST 2016, time=2016-09-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1694327, encodeId=1994169432e1d, content=<a href='/topic/show?id=9b558805847' target=_blank style='color:#2F92EE;'>#菌斑#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88058, encryptionId=9b558805847, topicName=菌斑)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7a9729602115, createdName=wrj0131, createdTime=Mon May 23 08:06:00 CST 2016, time=2016-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1824603, encodeId=1f6e1824603cb, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Fri Oct 02 01:06:00 CST 2015, time=2015-10-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1725860, encodeId=77801e258606f, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Sun Mar 20 19:06:00 CST 2016, time=2016-03-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=29400, encodeId=d57a29400ba, content=看看, beContent=null, objectType=article, channel=null, level=null, likeNumber=103, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ee0d1618732, createdName=huaxipanxing, createdTime=Thu Jun 25 23:45:00 CST 2015, time=2015-06-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27879, encodeId=31e52e87929, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=147, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:37:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27876, encodeId=fa3c2e8766f, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27877, encodeId=ec702e877fb, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=108, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27878, encodeId=d55e2e878a8, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27344, encodeId=6d732e344fd, content=很多疾病都会影响口腔微生物, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96871613250, createdName=medcardio, createdTime=Fri Jun 12 19:21:00 CST 2015, time=2015-06-12, status=1, ipAttribution=)]
    2016-03-20 feather89
  5. [GetPortalCommentsPageByObjectIdResponse(id=125050, encodeId=cd8f12505041, content=好文章,还会关注, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Tue Sep 13 11:51:00 CST 2016, time=2016-09-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1694327, encodeId=1994169432e1d, content=<a href='/topic/show?id=9b558805847' target=_blank style='color:#2F92EE;'>#菌斑#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88058, encryptionId=9b558805847, topicName=菌斑)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7a9729602115, createdName=wrj0131, createdTime=Mon May 23 08:06:00 CST 2016, time=2016-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1824603, encodeId=1f6e1824603cb, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Fri Oct 02 01:06:00 CST 2015, time=2015-10-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1725860, encodeId=77801e258606f, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Sun Mar 20 19:06:00 CST 2016, time=2016-03-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=29400, encodeId=d57a29400ba, content=看看, beContent=null, objectType=article, channel=null, level=null, likeNumber=103, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ee0d1618732, createdName=huaxipanxing, createdTime=Thu Jun 25 23:45:00 CST 2015, time=2015-06-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27879, encodeId=31e52e87929, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=147, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:37:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27876, encodeId=fa3c2e8766f, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27877, encodeId=ec702e877fb, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=108, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27878, encodeId=d55e2e878a8, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27344, encodeId=6d732e344fd, content=很多疾病都会影响口腔微生物, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96871613250, createdName=medcardio, createdTime=Fri Jun 12 19:21:00 CST 2015, time=2015-06-12, status=1, ipAttribution=)]
    2015-06-25 huaxipanxing

    看看

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=125050, encodeId=cd8f12505041, content=好文章,还会关注, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Tue Sep 13 11:51:00 CST 2016, time=2016-09-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1694327, encodeId=1994169432e1d, content=<a href='/topic/show?id=9b558805847' target=_blank style='color:#2F92EE;'>#菌斑#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88058, encryptionId=9b558805847, topicName=菌斑)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7a9729602115, createdName=wrj0131, createdTime=Mon May 23 08:06:00 CST 2016, time=2016-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1824603, encodeId=1f6e1824603cb, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Fri Oct 02 01:06:00 CST 2015, time=2015-10-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1725860, encodeId=77801e258606f, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Sun Mar 20 19:06:00 CST 2016, time=2016-03-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=29400, encodeId=d57a29400ba, content=看看, beContent=null, objectType=article, channel=null, level=null, likeNumber=103, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ee0d1618732, createdName=huaxipanxing, createdTime=Thu Jun 25 23:45:00 CST 2015, time=2015-06-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27879, encodeId=31e52e87929, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=147, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:37:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27876, encodeId=fa3c2e8766f, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27877, encodeId=ec702e877fb, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=108, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27878, encodeId=d55e2e878a8, content=!, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://api.medsci.cn/resource/upload/20150526/IMG5564536F3C41D5237.jpg, createdBy=4c0c1612254, createdName=summer474cn, createdTime=Wed Jun 17 08:36:00 CST 2015, time=2015-06-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=27344, encodeId=6d732e344fd, content=很多疾病都会影响口腔微生物, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96871613250, createdName=medcardio, createdTime=Fri Jun 12 19:21:00 CST 2015, time=2015-06-12, status=1, ipAttribution=)]
    2015-06-17 summer474cn

    0

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    2015-06-17 summer474cn

    0

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    2015-06-17 summer474cn

    0

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    2015-06-17 summer474cn

    0

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    2015-06-12 medcardio

    很多疾病都会影响口腔微生物

    0

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