Methods Mol Biol:从人诱导的多能干细胞产生功能性视网膜色素上皮细胞综述

2018-11-22 MedSci MedSci原创

德国雷根斯堡雷根斯堡大学医院眼科的Brandl C近日在Methods Mol Biol杂志上发表了一篇综述文章。在这篇文章中,作者详细描述了iPSC衍生的RPE单层细胞诱导过程,对它们的增殖和低温保存的方案也进行了讨论。同时,通过测量上皮抗性能力,来对功能性细胞分化进行监测。

德国雷根斯堡雷根斯堡大学医院眼科的Brandl C近日在Methods Mol Biol杂志上发表了一篇综述文章。在这篇文章中,作者详细描述了iPSC衍生的RPE单层细胞诱导过程,对它们的增殖和低温保存的方案也进行了讨论。同时,通过测量上皮抗性能力,来对功能性细胞分化进行监测。

通过诱导多能干细胞(iPSC)技术可以获得其他方法不易获得的眼内细胞,这使得研究者可以在退行性眼病中进行疾病建模、药物筛选,以及进行细胞治疗等。将iPSC直接分化为视网膜色素上皮细胞(RPE)是特别简单的,并且已经证明iPSC衍生的RPE细胞可以产生单一的功能性细胞群体,这些细胞群体具有和自然状态RPE相似的许多特征。

总之,这篇文章对诱导多能干细胞(iPSC)向视网膜色素上皮细胞(RPE)分化进行了总结,对眼科临床治疗提供了一种可行的方案。

原文出处:

Brandl, C., Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells. Methods Mol Biol, 2019. 1834: p. 87-94.

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    2018-12-30 一闲
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    2019-09-12 sunylz
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encryptionId=22a1328e7e5, topicName=功能性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=570f404, createdName=xlxchina, createdTime=Fri Jun 21 05:59:00 CST 2019, time=2019-06-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1810989, encodeId=122d18109897c, content=<a href='/topic/show?id=560e86e092b' target=_blank style='color:#2F92EE;'>#色素上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=86709, encryptionId=560e86e092b, topicName=色素上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=570f404, createdName=xlxchina, createdTime=Fri Aug 09 20:59:00 CST 2019, time=2019-08-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1767568, encodeId=5c801e67568dd, content=<a href='/topic/show?id=64f5343e2f' target=_blank style='color:#2F92EE;'>#Biol#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3437, encryptionId=64f5343e2f, topicName=Biol)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=446937890434, createdName=12498ebem31暂无昵称, createdTime=Sat Jul 27 15:59:00 CST 2019, time=2019-07-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1918413, encodeId=3bf71918413c7, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Thu Oct 17 11:59:00 CST 2019, time=2019-10-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1270139, encodeId=3cd712e013935, content=<a href='/topic/show?id=979690e33bd' target=_blank style='color:#2F92EE;'>#视网膜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90733, encryptionId=979690e33bd, topicName=视网膜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=742793, createdName=zutt, createdTime=Sat Nov 24 11:59:00 CST 2018, time=2018-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1533880, encodeId=6d31153388063, content=<a href='/topic/show?id=4be9200308c' target=_blank style='color:#2F92EE;'>#上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=20030, encryptionId=4be9200308c, topicName=上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3a5d12492718, createdName=lizhou0208, createdTime=Sat Nov 24 11:59:00 CST 2018, time=2018-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1568421, encodeId=85821568421b1, content=<a href='/topic/show?id=9fec9080901' target=_blank style='color:#2F92EE;'>#视网膜色素上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90809, encryptionId=9fec9080901, topicName=视网膜色素上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b1be15374853, createdName=wetgdt, createdTime=Sat Nov 24 11:59:00 CST 2018, time=2018-11-24, status=1, ipAttribution=)]
    2019-06-21 xlxchina
  5. [GetPortalCommentsPageByObjectIdResponse(id=1896424, encodeId=4eb91896424fc, content=<a href='/topic/show?id=4a391158248' target=_blank style='color:#2F92EE;'>#MET#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11582, encryptionId=4a391158248, topicName=MET)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=18fc139, createdName=一闲, createdTime=Sun Dec 30 09:59:00 CST 2018, time=2018-12-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1810961, encodeId=ab4b181096143, content=<a href='/topic/show?id=bf4486e08be' target=_blank style='color:#2F92EE;'>#色素上皮#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=86708, encryptionId=bf4486e08be, topicName=色素上皮)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=73a3403, 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status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1568421, encodeId=85821568421b1, content=<a href='/topic/show?id=9fec9080901' target=_blank style='color:#2F92EE;'>#视网膜色素上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90809, encryptionId=9fec9080901, topicName=视网膜色素上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b1be15374853, createdName=wetgdt, createdTime=Sat Nov 24 11:59:00 CST 2018, time=2018-11-24, status=1, ipAttribution=)]
  6. [GetPortalCommentsPageByObjectIdResponse(id=1896424, encodeId=4eb91896424fc, content=<a href='/topic/show?id=4a391158248' target=_blank style='color:#2F92EE;'>#MET#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11582, encryptionId=4a391158248, topicName=MET)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=18fc139, createdName=一闲, createdTime=Sun Dec 30 09:59:00 CST 2018, time=2018-12-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1810961, encodeId=ab4b181096143, content=<a href='/topic/show?id=bf4486e08be' target=_blank style='color:#2F92EE;'>#色素上皮#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=86708, encryptionId=bf4486e08be, topicName=色素上皮)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=73a3403, 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  7. 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status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1568421, encodeId=85821568421b1, content=<a href='/topic/show?id=9fec9080901' target=_blank style='color:#2F92EE;'>#视网膜色素上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90809, encryptionId=9fec9080901, topicName=视网膜色素上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b1be15374853, createdName=wetgdt, createdTime=Sat Nov 24 11:59:00 CST 2018, time=2018-11-24, status=1, ipAttribution=)]
    2019-10-17 sunylz
  8. 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status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1568421, encodeId=85821568421b1, content=<a href='/topic/show?id=9fec9080901' target=_blank style='color:#2F92EE;'>#视网膜色素上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90809, encryptionId=9fec9080901, topicName=视网膜色素上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b1be15374853, createdName=wetgdt, createdTime=Sat Nov 24 11:59:00 CST 2018, time=2018-11-24, status=1, ipAttribution=)]
    2018-11-24 zutt
  9. 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