Clin Oral Invest: 表皮链球菌的诱导表面蛋白粘附于钛种植体基质

2018-08-25 MedSci MedSci原创

表皮葡萄球菌(Staphylococcus epidermidis)作为主要的定植物,与(牙)种植体的感染(即种植体周围炎)密切相关,但对该细菌的表面蛋白质组知之甚少。为了鉴定细菌粘附素,本研究调查了直接粘附于钛种植体基质上的表皮葡萄球菌的表面蛋白质组。

表皮葡萄球菌(Staphylococcus epidermidis)作为主要的定植物,与(牙)种植体的感染(即种植体周围炎)密切相关,但对该细菌的表面蛋白质组知之甚少。为了鉴定细菌粘附素,本研究调查了直接粘附于钛种植体基质上的表皮葡萄球菌的表面蛋白质组。

表皮葡萄球菌菌株ATTC 35984浮游或在钛种植体上培养。通过变溶菌素消化分离表面蛋白质组,并通过2D凝胶电泳分离蛋白质以仅分离高表达的蛋白质。通过银染色显现蛋白质斑点,并通过质谱法鉴定蛋白质。

结果:对钛上表皮葡萄球菌的表面蛋白质组分析,进而鉴定了六种表达的蛋白质。三种蛋白质在钛种植体上高度表达,包括积累相关蛋白Q8CQD9。这些特定蛋白质可能是种植体周围生物膜中细菌的潜在致病因子。

此研究首次发现了表皮葡萄球菌表面蛋白,这些蛋白在粘附于钛种植体材料后表达。

原始出处:

R. Burgers, et al. Induced surface proteins of Streptococcus epidermidis adhering to titanium implant substrata. Clinical Oral Investigations. 2018 September. doi:10.1007/s00784-018-2508-9

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    2018-11-12 小几洁
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    2019-07-02 huangdf
  5. [GetPortalCommentsPageByObjectIdResponse(id=2051222, encodeId=18c7205122256, content=<a href='/topic/show?id=95f8e042dd' target=_blank style='color:#2F92EE;'>#EST#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7042, encryptionId=95f8e042dd, topicName=EST)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e9a6357, createdName=xiangyuzhou971, createdTime=Thu Dec 20 23:06:00 CST 2018, time=2018-12-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1757114, encodeId=eac71e5711418, content=<a href='/topic/show?id=103b421e0e8' target=_blank style='color:#2F92EE;'>#基质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42170, encryptionId=103b421e0e8, topicName=基质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=281337296540, createdName=ms7899726347904398, createdTime=Sun Nov 04 23:06:00 CST 2018, time=2018-11-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2020468, encodeId=dad520204681a, content=<a href='/topic/show?id=1584134451c' target=_blank style='color:#2F92EE;'>#Oral#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13445, encryptionId=1584134451c, topicName=Oral)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIpeueeYB1kem1yfEZrSYKBdZBoqac9USf0RxIgRICBIyDn4licyiaiakNnobeCDVpvwqaWgwTNeLRHA/132, createdBy=bb5e2500127, createdName=小几洁, createdTime=Mon Nov 12 19:06:00 CST 2018, time=2018-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701968, encodeId=c6381e0196876, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Tue Jul 02 22:06:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1679712, encodeId=b81b16e97124b, content=<a href='/topic/show?id=d4ac9639efa' target=_blank style='color:#2F92EE;'>#钛#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96397, encryptionId=d4ac9639efa, topicName=钛)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=927227807332, createdName=30397613, createdTime=Fri Apr 05 21:06:00 CST 2019, time=2019-04-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343811, encodeId=abb81343811a8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Mon Aug 27 12:06:00 CST 2018, time=2018-08-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1624064, encodeId=0082162406453, content=<a href='/topic/show?id=69699666026' target=_blank style='color:#2F92EE;'>#链球菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96660, encryptionId=69699666026, topicName=链球菌)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=787f20641298, createdName=liuquanmn, createdTime=Mon Aug 27 12:06:00 CST 2018, time=2018-08-27, status=1, ipAttribution=)]
    2019-04-05 30397613
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  7. [GetPortalCommentsPageByObjectIdResponse(id=2051222, encodeId=18c7205122256, content=<a href='/topic/show?id=95f8e042dd' target=_blank style='color:#2F92EE;'>#EST#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7042, encryptionId=95f8e042dd, topicName=EST)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e9a6357, createdName=xiangyuzhou971, createdTime=Thu Dec 20 23:06:00 CST 2018, time=2018-12-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1757114, encodeId=eac71e5711418, content=<a href='/topic/show?id=103b421e0e8' target=_blank style='color:#2F92EE;'>#基质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42170, encryptionId=103b421e0e8, topicName=基质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=281337296540, createdName=ms7899726347904398, createdTime=Sun Nov 04 23:06:00 CST 2018, time=2018-11-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2020468, encodeId=dad520204681a, content=<a href='/topic/show?id=1584134451c' target=_blank style='color:#2F92EE;'>#Oral#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13445, encryptionId=1584134451c, topicName=Oral)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIpeueeYB1kem1yfEZrSYKBdZBoqac9USf0RxIgRICBIyDn4licyiaiakNnobeCDVpvwqaWgwTNeLRHA/132, createdBy=bb5e2500127, createdName=小几洁, createdTime=Mon Nov 12 19:06:00 CST 2018, time=2018-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1701968, encodeId=c6381e0196876, content=<a href='/topic/show?id=04b9e4784aa' target=_blank style='color:#2F92EE;'>#种植#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74784, encryptionId=04b9e4784aa, topicName=种植)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=47e130405619, createdName=huangdf, createdTime=Tue Jul 02 22:06:00 CST 2019, time=2019-07-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1679712, encodeId=b81b16e97124b, content=<a href='/topic/show?id=d4ac9639efa' target=_blank style='color:#2F92EE;'>#钛#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96397, encryptionId=d4ac9639efa, topicName=钛)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=927227807332, createdName=30397613, createdTime=Fri Apr 05 21:06:00 CST 2019, time=2019-04-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1343811, encodeId=abb81343811a8, content=<a href='/topic/show?id=d512e47853f' target=_blank style='color:#2F92EE;'>#种植体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=74785, encryptionId=d512e47853f, topicName=种植体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/TnzXXl41yf6V8fHrDb5JdtxOgpaY6vplUhHtL8BzDh1xMtcap9H1IOlE8umxPGovhIuVkeGTY26eNUFCFaOheQ/132, createdBy=29702500197, createdName=ms6279672939590805, createdTime=Mon Aug 27 12:06:00 CST 2018, time=2018-08-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1624064, encodeId=0082162406453, content=<a href='/topic/show?id=69699666026' target=_blank style='color:#2F92EE;'>#链球菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96660, encryptionId=69699666026, topicName=链球菌)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=787f20641298, createdName=liuquanmn, createdTime=Mon Aug 27 12:06:00 CST 2018, time=2018-08-27, status=1, ipAttribution=)]