Arch Med Res：葡萄糖转运蛋白1促癌细胞转移机制

葡萄糖转运蛋白是一类专门运送葡萄糖的蛋白称为葡萄糖转运蛋白。它一共分4种：葡萄糖转运蛋白1～4(Glut1～4)。Glut4是一种类胰岛素敏感型葡萄糖运输载体，它与2型糖尿病密切相关，细胞中Glut4表达的减少以及其转位的障碍都是引发糖尿病的重要因素。

早期研究发现葡萄糖转运蛋白-1（Glut-1）与恶性肿瘤的生物学行为密切相关。然而，没有任何证据阐明Glut -1的过度表达对癌细胞侵袭转移机制。研究人员推测，Glut -1调节膜型金属蛋白酶-1（MT1-MMP）和基质金属蛋白酶-2（MMP-2）的表达促进癌细胞侵袭运动。

采用RT-PCR分析Glut -1、MMP-2、β-肌动蛋白、MT1-MMP的表达。Western印迹法检测Glut -1蛋白、MMP-2、MT1-MMP的表达。结果发现在Glut -1和MMP-2 mRNA和蛋白水平，两者共同表达于喉癌Hep-2喉癌细胞株中。

而在Hep-2喉癌细胞株转染Glut -1反义寡核苷酸（AS-ODN）后，MMP-2 mRNA和蛋白表达以及Glut -1 mRNA和蛋白表达出现下降。研究数据证实Glut -1和MMP-2的共表达在Hep-2喉癌细胞中，Glut -1可调节MMP-2和MT1-MMP的表达。

编译自：Effect on the Expression of MMP-2, MT-MMP in Laryngeal Carcinoma Hep-2 Cell by Antisense Glucose Transporter-1

doi:10.1016/j.arcmed.2012.07.003
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PMID:

Effect on the Expression of MMP-2, MT-MMP in Laryngeal Carcinoma Hep-2 Cell by Antisense Glucose Transporter-1

Ying-Ying Xua, Yang-Yang Baoa, Shui-Hong Zhoua, Jun Fanb

Background and Aims

Glucose transporter protein-1 (Glut-1) is correlated with biological behaviors of malignant tumors. However, there was no evidence that overexpression of Glut-1 mechanistically lead to invasion or metastasis of cancer cells. We hypothesized that Glut-1 regulates the expression of membrane type 1-MMP (MT1-MMP) and matrix metalloproteinase-2 (MMP-2).

Methods

Analysis of the expression of Glut-1, MMP-2, β-actin, and MT1-MMP was performed using RT-PCR. Expression of Glut-1 protein, MMP-2, and MT1-MMP was detected by Western blotting.

Results

At mRNA and protein levels, Glut-1 and MMP-2 were co-expressed in the Hep-2 laryngeal carcinoma cell line. After transfection, Glut-1 antisense oligodeoxynucleotide (AS-ODN) decreased the expression of MMP-2 mRNA and protein as well as Glut-1 mRNA and protein. Glut-1 AS-ODN also decreased the expression of MT1-MMP mRNA.

Conclusions

Co-expression of Glut-1 and MMP-2 in Hep-2 laryngeal carcinoma cells and Glut-1 may regulate MMP-2 and MT1-MMP expression.