Neural Regen Res. ：四种细胞与脑脊液联合培养液中神经营养因子的表达

一项关于“Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium ”的研究显示，将人胚嗅鞘细胞、人许旺细胞、人羊膜上皮细胞和人血管内皮细胞在含脑脊液的完全培养基中联合培养。结果证实，人胚嗅鞘细胞、人许旺细胞、人羊膜上皮细胞和人血管内皮细胞在含脑脊液培养基中联合培养可促进培养液中神经生长因子、脑源性神经营养因子和胶质源性神经营养因子的表达。脊髓损伤后减少移植部位脑脊液的外渗对移植细胞生存及分泌神经营养因子有益。

相关研究成果发表在《中国神经再生研究（英文版）》杂志于2012年12月36期出版的杂志上。

doi:10.3969/j.issn.1673-5374.2012.36.008
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Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium

Sanjiang Feng1, Minghua Zhuang1, Rui Wu2

The present study co-cultured human embryonic olfactory ensheathing cells, human Schwann cells, human amniotic epithelial cells and human vascular endothelial cells in complete culture mediumcontaining cerebrospinal fluid. Enzyme linked immunosorbent assay was used to detect nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor secretion in the supernatant of co-cultured cells. Results showed that the number of all cell types reached a peak at 7–10 days, and the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor peaked at 9 days. Levels of secreted nerve growth factor were four-fold higher than brain-derived neurotrophic factor, which was three-fold higher than glial cell line-derived neurotrophic factor. Increasing concentrations of cerebrospinal fluid (10%, 20% and 30%) in the growth medium caused a decrease of neurotrophic factor secretion. Results indicated co-culture of human embryonic olfactory ensheathing cells, human Schwann cells, human amniotic epithelial cells and human vascular endothelial cells improved the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor. The reduction of cerebrospinal fluid extravasation at the transplant site after spinal cord injury is beneficial for the survival and secretion of neurotrophic factors from transplanted cells.