Biomedica:瓣膜置换术患者瓣膜中的微生物是否来源于牙周微生物

2018-01-29 MedSci MedSci原创

牙周炎是影响牙齿支持组织的慢性感染性疾病,与多种全身性疾病(包括心血管疾病)的发生有关。微生物学研究有助于检测龈下和心血管样品中的微生物。本研究旨在描述瓣膜置换手术的患者的心脏瓣膜微生物和牙周微生物群。使用两相培养基,添加了血琼脂和含抗生素的胰蛋白酶大豆琼脂,分析了30个龈下和瓣膜组织样品。对瓣膜组织进行常规PCR检测分析。结果显示,从牙周袋分离的牙周致病菌分别为:具核梭杆菌50%,中间普氏菌40

牙周炎是影响牙齿支持组织的慢性感染性疾病,与多种全身性疾病(包括心血管疾病)的发生有关。微生物学研究有助于检测龈下和心血管样品中的微生物。本研究旨在描述瓣膜置换手术的患者的心脏瓣膜微生物和牙周微生物群。

使用两相培养基,添加了血琼脂和含抗生素的胰蛋白酶大豆琼脂,分析了30个龈下和瓣膜组织样品。对瓣膜组织进行常规PCR检测分析。

结果显示,从牙周袋分离的牙周致病菌分别为:具核梭杆菌50%,中间普氏菌40%,直肠弯曲杆菌40%,艾肯耐克菌36.7%,革兰氏阴性肠杆菌36.7%,牙龈卟啉单胞菌(33.3%)和真杆菌属33.3%。从主动脉瓣分离的病原菌有痤疮丙酸杆菌(12%),革兰氏阴性肠杆菌(8%),类杆菌(4%)和梭菌梭状芽孢杆菌(4%),从二尖瓣研究人员分离了痤疮丙酸杆菌和拜氏梭菌。常规PCR检测口腔病原体为阴性,仅在两个样品中检测到细菌DNA。

总之,该研究结果表明,接受心脏瓣膜置换手术的患者的牙周微生物群由与口外组织感染相关的革兰氏阴性细菌种类组成。然而,没有证据表明牙周病原体存在于瓣膜组织中,因为即使瓣膜和龈下样本存在革兰氏阴性肠杆菌,也无法确保二者来源相同。

原始出处:


Moreno S, Parra B, Botero JE, et al., Periodontal microbiota and microorganisms isolated from heart valves in patients undergoing valve replacement surgery in a clinic in Cali, Colombia. Biomedica. 2017 Dec 1;37(4):516-525. doi: 10.7705/biomedica.v37i4.3232.

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    2018-09-18 jeanqiuqiu
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    2018-05-11 sunylz
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    2018-07-16 yxch48
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  5. 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  6. 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target=_blank style='color:#2F92EE;'>#置换术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79664, encryptionId=48fee966468, topicName=置换术)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Wed Jan 31 10:49:00 CST 2018, time=2018-01-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1583551, encodeId=13d715835517e, content=<a href='/topic/show?id=508d68e698c' target=_blank style='color:#2F92EE;'>#瓣膜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=20, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=68769, encryptionId=508d68e698c, topicName=瓣膜)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=58dc16986228, createdName=12498e5fm17(暂无昵称), createdTime=Wed Jan 31 10:49:00 CST 2018, time=2018-01-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1608492, encodeId=a2931608492ed, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=19, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Wed Jan 31 10:49:00 CST 2018, time=2018-01-31, status=1, ipAttribution=)]
    2018-02-17 天涯183

    非常好的文章.学习了

    0

  7. 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