Front Microbiol:氮磷对大肠杆菌生物膜形成的影响

2018-04-03 MedSci MedSci原创

在这项研究中,研究人员旨在确定氮(N)和磷(P)对大肠杆菌多重耐药性和生物膜形成能力的影响。检测生物膜形成相关基因的表达以建立基因型和表型之间的相关性。加入不同浓度的N和P形成一个对照组和四个处理组。玻璃管法用于确定生物膜形成能力。实时PCR用于检测大肠杆菌中六种生物膜形成相关基因的mRNA丰度。结果,没有从对照组中分离出耐药菌株,同时,治疗组的多药耐药率较高。在所有处理组中检测到生物膜相关基因l

在这项研究中,研究人员旨在确定氮(N)和磷(P)对大肠杆菌多重耐药性和生物膜形成能力的影响。

检测生物膜形成相关基因的表达以建立基因型和表型之间的相关性。加入不同浓度的N和P形成一个对照组和四个处理组。玻璃管法用于确定生物膜形成能力。实时PCR用于检测大肠杆菌中六种生物膜形成相关基因的mRNA丰度。

结果,没有从对照组中分离出耐药菌株,同时,治疗组的多药耐药率较高。在所有处理组中检测到生物膜相关基因luxS,flhD,fliA,motA和fimH的表达,然而,没有mqsR的表达。 luxS,flhD,fliA,motA和fimH的表达与N、P浓度,以及不同组间多药耐药的表现和持续时间明显相关。

总的来说,这项研究的结果表明,生物膜形成能力在添加N和P在大肠杆菌后形成多药物抗性中起关键作用。

原始出处:

Chen XP, Ali L, et al., Biofilm Formation Plays a Role in the Formation of Multidrug-Resistant Escherichia coli Toward Nutrients in Microcosm Experiments. Front Microbiol. 2018 Mar 2;9:367. doi: 10.3389/fmicb.2018.00367. eCollection 2018.

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    2018-09-29 xjy02
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    2018-09-22 sunylz
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    2018-04-03 清风拂面

    谢谢分享学习

    0

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