J Dent Res:白色念珠菌在牙科丙烯酸表面的早期黏附

2017-07-07 MedSci MedSci原创

义齿相关性口炎是一种常见的念珠菌感染,可能会引起疼痛或其他部位感染源。由于聚(甲基丙烯酸甲酯)(PMMA)仍然是用于制作义齿的主要材料,本研究的目的是通过使用原子力显微镜(AFM)单细胞力光谱学(SCFS)技术来评估白色念珠菌细胞对PMMA表面的粘附作用。

义齿相关性口炎是一种常见的念珠菌感染,可能会引起疼痛或其他部位感染源。由于聚(甲基丙烯酸甲酯)(PMMA)仍然是用于制作义齿的主要材料,本研究的目的是通过使用原子力显微镜(AFM)单细胞力光谱学(SCFS)技术来评估白色念珠菌细胞对PMMA表面的粘附作用。

对于实验,无尖AFM悬臂用PMMA微球进行功能化,并探测固定在生物聚合物涂覆的基板上的白色念珠菌。实验室菌株和白色念珠菌的临床分离物均用于SCFS实验。白色念珠菌的扫描电子显微镜(SEM)和AFM成像证实了两种菌株的多态性,这取决于生长培养条件。 AFM力谱分析结果表明,白色念珠菌对PMMA的粘附是形态依赖性的,因为菌丝管比酵母细胞粘附力高(P <0.05)。白色念珠菌发芽的母细胞是非粘附的,这与在管区域中观察到的粘附增加形成对比。与实验室菌株相比,临床分离物的粘附力高。临床分离物还增加了血液中的存活率,降低了对补体调理作用的敏感性,提供了可能影响毒力的念珠菌宿主相互作用中应变依赖性差异的额外证据。总之,PMMA修饰的AFM探针已被证明是一种可靠的检测白色念珠菌对丙烯酸酯表面的粘附作用的技术。

原始出处:

S. Aguayo, H. Marshall, J. Pratten, et al. Early Adhesion of Candida albicans onto Dental Acrylic Surfaces. Journal of Dental Research. 2017 July. doi: https://doi.org/10.1177/0022034517706354

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    2017-07-09 xzw115
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    2017-07-07 130****4638

    学习了受益匪浅

    0

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    2017-07-07 189****7206

    学习了谢谢分享

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