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J Periodontol:振动通过环氧合酶途径加强hpdl细胞中PGE 2、IL-6和IL-8的表达

2018-05-28 MedSci MedSci原创

振动正畸牙移动环氧合酶途径
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虽然振动与正畸力结合可能会加速正畸牙齿的移动，但对调节相关细胞反应的机制知之甚少。本研究的目的是研究机械振动联合压力是否通过环氧合酶途径调控人牙周膜细胞中COX-2 / PGE2，IL-6和IL-8的mRNA和蛋白表达。

虽然振动与正畸力结合可能会加速正畸牙齿的移动，但对调节相关细胞反应的机制知之甚少。本研究的目的是研究机械振动联合压力是否通过环氧合酶途径调控人牙周膜细胞中COX-2 / PGE2，IL-6和IL-8的mRNA和蛋白表达。

将原代培养的hPDL细胞暴露于机械振动，压缩力或机械振动和压缩力下24，48，72小时。使用定量实时聚合酶链式反应（qPCR）测定COX-2，IL-6，IL-8，RANKL和OPG mRNA表达。通过酶联免疫吸附测定（ELISA）定量PGE2，IL-6和IL-8蛋白表达。用吲哚美辛处理hPDL细胞PGE2，IL-6和IL-8的表达确定是否是环氧合酶依赖性途径。为了确定PGE 2是否刺激hPDL细胞表达IL-6和IL-8，将外源性PGE 2加入到培养基中。

结果：机械振动和压缩力协同作用可上调RANKL / OPG比率，COX-2 / PGE2，IL-6和IL-8的mRNA和蛋白表达。吲哚美辛显着减弱了用压缩力或机械振动和压缩力刺激的细胞中PGE2，IL-6和IL-8表达的增加。另外，外源性PGE 2增加了hPDL细胞中的IL-6和IL-8 mRNA和蛋白质表达。

机械振动可通过环氧合酶途径的机制增强正畸牙移动期间压缩侧的牙槽骨吸收。

原始出处：

Phusuntornsakul P， et al. Vibration enhances PGE2 ， IL-6 and IL-8 expression in compressed hPDL cells via cyclooxygenase pathway. Journal of Periodontology. 2018 May. doi：10.1002/JPER.17-0653

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    2019-01-31 feather89

    #PE#

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    2018-05-30 kzlchina

    #IL-6#

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    2018-05-30 respect

    #IL-8#

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authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/05/13/e51eda1902bf3197b7b2a48e1ba1e39c.jpg, createdBy=6a672335244, createdName=724765917_36481625, createdTime=Tue May 29 01:12:41 CST 2018, time=2018-05-29, status=1, ipAttribution=)]
    2018-05-29 龙胆草

    学习谢谢分享

    0

  5. 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    2018-05-29 1e17414fm02(暂无匿称)

    学习

    0

  6. 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    2018-05-29 1209e435m98(暂无昵称)

    学习了.谢谢分享

    0

  7. 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    2018-05-29 1dd8c52fm63(暂无匿称)

    了解了解.学习学习

    0

  8. 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replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7c592194322, createdName=1209e435m98(暂无昵称), createdTime=Tue May 29 06:50:10 CST 2018, time=2018-05-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=319513, encodeId=a116319513fe, content=了解了解.学习学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Tue May 29 05:30:52 CST 2018, time=2018-05-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=319508, encodeId=1094319508b2, content=好好好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Tue May 29 05:13:34 CST 2018, time=2018-05-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=319507, encodeId=84dd31950e7c, content=学习了.获益匪浅.感谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/02/27/c53a212859edcad4f60a54feffff50bb.jpg, createdBy=67cf1525321, createdName=13718711b3m, createdTime=Tue May 29 03:46:03 CST 2018, time=2018-05-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=319505, encodeId=29e9319505f6, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/05/13/e51eda1902bf3197b7b2a48e1ba1e39c.jpg, createdBy=6a672335244, createdName=724765917_36481625, createdTime=Tue May 29 01:12:41 CST 2018, time=2018-05-29, status=1, ipAttribution=)]
    2018-05-29 yjs木玉

    好好好好好好好好

    0

  9. 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authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/05/13/e51eda1902bf3197b7b2a48e1ba1e39c.jpg, createdBy=6a672335244, createdName=724765917_36481625, createdTime=Tue May 29 01:12:41 CST 2018, time=2018-05-29, status=1, ipAttribution=)]
    2018-05-29 13718711b3m

    学习了.获益匪浅.感谢分享

    0

  10. 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authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/05/13/e51eda1902bf3197b7b2a48e1ba1e39c.jpg, createdBy=6a672335244, createdName=724765917_36481625, createdTime=Tue May 29 01:12:41 CST 2018, time=2018-05-29, status=1, ipAttribution=)]
    2018-05-29 724765917_36481625

    学习了

    0

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