Wounds.:Gelam蜂蜜促进角膜上皮细胞增殖期的潜力!

2017-07-06 cuiguizhong MedSci原创

用0.0015%GH处理的培养基增加了离体角膜上皮细胞增殖,同时保留了其表型特征。本研究证实了GH型局部治疗对超声角膜损伤的潜在发展。

近日,来自马来西亚科邦沙医科大学生理系的Hui CK团队在Wounds.杂志上发表了他们的最近研究:Gelam蜂蜜促进角膜上皮细胞增殖期的潜力。

在本项研究中,作者们调查了Gelam蜂蜜(GH)促进离体角膜上皮细胞(CECs)增殖及其对表型特征的影响的潜在益处。

他们从兔角膜(n = 6)中分离角膜上皮细胞。通过MTT(3- [4,5-二甲基噻唑基-2] -2,5-二苯基四唑溴化物)测定法测定基础培养基(BM)和角膜培养基(CM)中离体角膜上皮细胞增殖的最佳剂量。通过相差显微镜、实时聚合酶链反应、免疫细胞化学和流式细胞技术分别评估了离体角膜上皮细胞的形态、基因和蛋白表达、细胞周期分析。

研究结果表明,在0.0015%GH补充培养基(BM + 0.0015%GH; CM + 0.0015%GH)中培养的角膜上皮细胞具有正常多边形形态的最佳增殖能力。 Gelam蜂蜜根据细胞质CK3蛋白表达增强细胞角蛋白3(CK3)基因表达,同时保持离体角膜上皮细胞的正常细胞周期。

由此,作者们得出结论:用0.0015%GH处理的培养基增加了离体角膜上皮细胞增殖,同时保留了其表型特征。本研究证实了GH型局部治疗对超声角膜损伤的潜在发展。

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    2018-03-07 lxg951
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    2017-07-08 snowpeakxu
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