Food Chem: 蜂蜜对前列腺癌细胞具有毒性且与MTT反应物互作

2017-10-07 AlexYang MedSci原创

蜂蜜是一种复杂的生物物质,包含有许多的糖类、酚类复合物和酶类。最近,有研究人员利用5种快速的和可检测的实验来测量蜂蜜体外细胞毒性。研究发现,蜂蜜对前列腺癌细胞PC3和DU145具有细胞学毒性。然而,细胞死亡的水平在不同试验中水平不同。MTT试验容易被MTT反应物的减少而影响,并且蜂蜜减少的糖类和酚类物质容易使得MTT试验结果不同。乳酸脱氢酶试验容易因蜂蜜氧化酵素辅助因子NADH而使得试验无效。磺酰

蜂蜜是一种复杂的生物物质,包含有许多的糖类、酚类复合物和酶类。最近,有研究人员利用5种快速的和可检测的实验来测量蜂蜜体外细胞毒性。

研究发现,蜂蜜对前列腺癌细胞PC3和DU145具有细胞学毒性。然而,细胞死亡的水平在不同试验中水平不同。MTT试验容易被MTT反应物的减少而影响,并且蜂蜜减少的糖类和酚类物质容易使得MTT试验结果不同。乳酸脱氢酶试验容易因蜂蜜氧化酵素辅助因子NADH而使得试验无效。磺酰罗丹明B试验能够给出确定的结果,但是只曾良蛋白含量,不能提供细胞死亡和剩余细胞的相关信息。台盼蓝试验和基于显微镜的碘化丙啶/赫斯特染色试验只评估了晚期膜透性数据。然而,碘化丙啶/赫斯特染色试验能够提供细胞死亡机制的形态学信息。最后,研究人员指出,磺酰罗丹明B试验和碘化丙啶/赫斯特染色试验的结合可以为蜂蜜的细胞学毒性提供最为准确的定量。

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    2017-10-09 yxch36
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    2017-10-09 ylz8405
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    2017-10-08 hhh678

    henhao

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