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F1000 Research：研究发现肉瘤中CIP2A表达调控新机制

2014-05-26 紫夕 医学论坛网

人纤维肉瘤JNK抑制剂肉瘤细胞
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澳大利亚和芬兰学者研究发现SP600125(JNK抑制剂)能抑制蛋白磷酸酶2A癌症抑制因子(CIP2A)的蛋白表达，其对表达时间和表达浓度均有限制作用。然而，利用特定小分子干扰RNA(siRNA)消耗c—JUN氨基末端激酶(JNK)1和JNK2激酶都不能降低CIP2A蛋白表达水平，因此需要通过特定方法证实使用激酶小分子抑制剂的治疗结果，例如使用两种不同的靶向特定siRNA。论文2014年8月在线发

澳大利亚和芬兰学者研究发现SP600125(JNK抑制剂)能抑制蛋白磷酸酶2A癌症抑制因子(CIP2A)的蛋白表达，其对表达时间和表达浓度均有限制作用。然而，利用特定小分子干扰RNA(siRNA)消耗c—JUN氨基末端激酶(JNK)1和JNK2激酶都不能降低CIP2A蛋白表达水平，因此需要通过特定方法证实使用激酶小分子抑制剂的治疗结果，例如使用两种不同的靶向特定siRNA。论文2014年8月在线发表于 F1000 Research 。

该研究利用小分子抑制剂对抗p38、MEK和JNK这三大信号通路，明确肉瘤细胞中调控CIP2A表达的通路，从而治疗人纤维肉瘤(HT1080)细胞。之后，利用激酶小分子干扰RNA(siRNA)核实结果。

结果如下：

与之前的观察一致，MEK通路小分子抑制剂(PD98059)降低CIP2A mRNA及蛋白表达。

有趣的是，JNK通路小分子抑制剂SP600125降低CIP2A肿瘤蛋白的mRNA和蛋白水平，而SB203580(p38激酶)抑制剂对HT1080细胞的CIP2A表达作用很小。

但是，JNK1或JNK2激酶所对应的siRNA并没有导致CIP2A表达增加。相反，用作阳性对照的两种不同的CIP2A siRNA则降低了HT1080细胞的JNK2表达。


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    2014-06-30 ms7019579240139182

    #F1000#

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  2. [GetPortalCommentsPageByObjectIdResponse(id=1947960, encodeId=95db194e9602b, content=<a href='/topic/show?id=060fe19851' target=_blank style='color:#2F92EE;'>#F1000#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7198, encryptionId=060fe19851, topicName=F1000)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/101296147/017ED6AFEB45F49165298113DDC0656A/100, createdBy=84182500174, createdName=ms7019579240139182, createdTime=Mon Jun 30 22:56:00 CST 2014, time=2014-06-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1753069, encodeId=b0681e5306912, content=<a href='/topic/show?id=6a57e33772b' target=_blank style='color:#2F92EE;'>#研究发现#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73377, encryptionId=6a57e33772b, topicName=研究发现)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7e1736602208, createdName=hanhaisha2020, createdTime=Wed Apr 08 22:56:00 CST 2015, time=2015-04-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557717, encodeId=6925155e7173f, content=<a href='/topic/show?id=d2601533982' target=_blank style='color:#2F92EE;'>#Research#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=15339, encryptionId=d2601533982, topicName=Research)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96ab14929432, createdName=yangshch, createdTime=Wed May 28 01:56:00 CST 2014, time=2014-05-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1984304, encodeId=cf221984304d6, content=<a href='/topic/show?id=4cfa902e9fe' target=_blank style='color:#2F92EE;'>#表达调控#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=90279, encryptionId=4cfa902e9fe, topicName=表达调控)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e572480, createdName=旅苦化文_208, createdTime=Mon Mar 16 17:56:00 CST 2015, time=2015-03-16, status=1, ipAttribution=)]
    2015-04-08 hanhaisha2020

    #研究发现#

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    2014-05-28 yangshch

    #Research#

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    2015-03-16 旅苦化文_208

    #表达调控#

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