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miRNA145抑制骨肉瘤细胞增殖

2012-06-29 鲍爱宁摘编 医学论坛网

miRNA145骨肉瘤
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　　最近，华中科技大学同济医学院附属协和医院骨科、南京医科大学第二附属医院骨科的研究人员的一项研究显示，miRNA145可以有效地抑制骨肉瘤细胞的增殖，促进细胞凋亡。 更多还原该研究发表于2012年第4期《中国癌症杂志》。 　　该研究旨在探讨miRNA145对骨肉瘤细胞增殖、细胞周期以及凋亡的影响。 　　研究人员用脂质体法将miRNA145真核表达质粒瞬时转染入人骨肉瘤细胞(MG63

　　最近，华中科技大学同济医学院附属协和医院骨科、南京医科大学第二附属医院骨科的研究人员的一项研究显示，miRNA145可以有效地抑制骨肉瘤细胞的增殖，促进细胞凋亡。 更多还原该研究发表于2012年第4期《中国癌症杂志》。

　　该研究旨在探讨miRNA145对骨肉瘤细胞增殖、细胞周期以及凋亡的影响。

　　研究人员用脂质体法将miRNA145真核表达质粒瞬时转染入人骨肉瘤细胞(MG63)，实验设置空白对照组、空载体转染组和miRNA145质粒转染组3组，转染后荧光显微镜下观察转染效率，实时定量RT-PCR检测各组细胞miRNA145的表达，MTT增殖实验检测各组细胞的增殖情况，流式细胞仪检测各组细胞周期及细胞凋亡率。

　　结果，转染后miRNA145质粒转染组细胞增殖速度明显下降，细胞周期停止在G1期之前，凋亡率增高，差异有统计学意义(P<0.05)。

　　



    

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  1. [GetPortalCommentsPageByObjectIdResponse(id=1930354, encodeId=4c7c1930354b0, content=<a href='/topic/show?id=22f61180211' target=_blank style='color:#2F92EE;'>#miR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=20, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11802, encryptionId=22f61180211, topicName=miR)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0a27288, createdName=smallant2002, createdTime=Thu Jul 26 10:05:00 CST 2012, time=2012-07-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1312242, encodeId=7e7f13122425c, content=<a href='/topic/show?id=e5571193321' target=_blank style='color:#2F92EE;'>#miRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11933, encryptionId=e5571193321, topicName=miRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e87d405, createdName=Homburg, createdTime=Sun Jul 01 06:05:00 CST 2012, time=2012-07-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1540861, encodeId=4f4a15408612d, content=<a href='/topic/show?id=ba2ee78630b' target=_blank style='color:#2F92EE;'>#细胞增殖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77863, encryptionId=ba2ee78630b, topicName=细胞增殖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f58613291856, createdName=晓辰, createdTime=Sun Jul 01 06:05:00 CST 2012, time=2012-07-01, status=1, ipAttribution=)]
    2012-07-26 smallant2002

    #miR#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1930354, encodeId=4c7c1930354b0, content=<a href='/topic/show?id=22f61180211' target=_blank style='color:#2F92EE;'>#miR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=20, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11802, encryptionId=22f61180211, topicName=miR)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0a27288, createdName=smallant2002, createdTime=Thu Jul 26 10:05:00 CST 2012, time=2012-07-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1312242, encodeId=7e7f13122425c, content=<a href='/topic/show?id=e5571193321' target=_blank style='color:#2F92EE;'>#miRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11933, encryptionId=e5571193321, topicName=miRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e87d405, createdName=Homburg, createdTime=Sun Jul 01 06:05:00 CST 2012, time=2012-07-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1540861, encodeId=4f4a15408612d, content=<a href='/topic/show?id=ba2ee78630b' target=_blank style='color:#2F92EE;'>#细胞增殖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77863, encryptionId=ba2ee78630b, topicName=细胞增殖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f58613291856, createdName=晓辰, createdTime=Sun Jul 01 06:05:00 CST 2012, time=2012-07-01, status=1, ipAttribution=)]
    2012-07-01 Homburg

    #miRNA#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1930354, encodeId=4c7c1930354b0, content=<a href='/topic/show?id=22f61180211' target=_blank style='color:#2F92EE;'>#miR#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=20, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11802, encryptionId=22f61180211, topicName=miR)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0a27288, createdName=smallant2002, createdTime=Thu Jul 26 10:05:00 CST 2012, time=2012-07-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1312242, encodeId=7e7f13122425c, content=<a href='/topic/show?id=e5571193321' target=_blank style='color:#2F92EE;'>#miRNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11933, encryptionId=e5571193321, topicName=miRNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e87d405, createdName=Homburg, createdTime=Sun Jul 01 06:05:00 CST 2012, time=2012-07-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1540861, encodeId=4f4a15408612d, content=<a href='/topic/show?id=ba2ee78630b' target=_blank style='color:#2F92EE;'>#细胞增殖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77863, encryptionId=ba2ee78630b, topicName=细胞增殖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f58613291856, createdName=晓辰, createdTime=Sun Jul 01 06:05:00 CST 2012, time=2012-07-01, status=1, ipAttribution=)]
    2012-07-01 晓辰

    #细胞增殖#

    0